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mole. genetics ch.12

QuestionAnswer
what is a plasmid? self-replicating, circular DNA molecules that are maintained at a characteristic number of copies
what are low-copy number plasmids? plasmids that are maintained at a constant quantity (usually 1-10 per cell)
what are high-copy number plasmids plasmids that exist in many copies per bacterium and may be segregated randomly; there are enough copies to ensure that each daughter cell gains enough plasmids through random distribution
what are episomes? plasmids that can integrate into the bacterial chromosome (main DNA)
how are plasmids segregated into daughter cells? par systems for low copy, and stochastic distribution for high copy
what is immunity (with respect to plasmids)? when plasmids maintain a selfish control over a bacterium, making it impossible for other types of plasmids to become established. (competition)
how is DNA replication accomplished in adenovirus at the end of their linear chromosome? synthesis of a new strand occurs at one end and finishes at the other, leaving the displaced strand as "free". this free strand is then replicated independently, forming a duplex origin by base pairing between short complementary sequences at the ends
what does the hairpin structure do?
what is rolling circle replication? unidirectional nucleic acid replication process where a circular DNA molecule serves as a template, producting long single-stranded DNA copes with tandem repeats.
what is strand displacement? process by which a new DNA strand grows by displacing the previous (homologous) strand of the duplex.
what is an F plasmid? circular, self-transmissible plasmid that enables conjugation
what is an F+ cell? a bacterium that contains the F plasmid, acting as a donot during conjugation
what is an F- cell? recipient bacterium that lacks the F plasmid
how does an F- cell become an F+ cell? bacterial conjugation
what is conjugation? a process in which two cells come in contact and transfer genetic material
what are pili? the bridge between 2 bacterium formed for conjugation
what is an Hfr cell? a bacterium that has an integrated F plasmid within its chromosome
how are mitochondria segregated during replication? they are segregated stochastically
how are mitochondrial genomes replicated? stochastically
Created by: angievelasco
 

 



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