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mole. genetics ch.12
| Question | Answer |
|---|---|
| what is a plasmid? | self-replicating, circular DNA molecules that are maintained at a characteristic number of copies |
| what are low-copy number plasmids? | plasmids that are maintained at a constant quantity (usually 1-10 per cell) |
| what are high-copy number plasmids | plasmids that exist in many copies per bacterium and may be segregated randomly; there are enough copies to ensure that each daughter cell gains enough plasmids through random distribution |
| what are episomes? | plasmids that can integrate into the bacterial chromosome (main DNA) |
| how are plasmids segregated into daughter cells? | par systems for low copy, and stochastic distribution for high copy |
| what is immunity (with respect to plasmids)? | when plasmids maintain a selfish control over a bacterium, making it impossible for other types of plasmids to become established. (competition) |
| how is DNA replication accomplished in adenovirus at the end of their linear chromosome? | synthesis of a new strand occurs at one end and finishes at the other, leaving the displaced strand as "free". this free strand is then replicated independently, forming a duplex origin by base pairing between short complementary sequences at the ends |
| what does the hairpin structure do? | |
| what is rolling circle replication? | unidirectional nucleic acid replication process where a circular DNA molecule serves as a template, producting long single-stranded DNA copes with tandem repeats. |
| what is strand displacement? | process by which a new DNA strand grows by displacing the previous (homologous) strand of the duplex. |
| what is an F plasmid? | circular, self-transmissible plasmid that enables conjugation |
| what is an F+ cell? | a bacterium that contains the F plasmid, acting as a donot during conjugation |
| what is an F- cell? | recipient bacterium that lacks the F plasmid |
| how does an F- cell become an F+ cell? | bacterial conjugation |
| what is conjugation? | a process in which two cells come in contact and transfer genetic material |
| what are pili? | the bridge between 2 bacterium formed for conjugation |
| what is an Hfr cell? | a bacterium that has an integrated F plasmid within its chromosome |
| how are mitochondria segregated during replication? | they are segregated stochastically |
| how are mitochondrial genomes replicated? | stochastically |