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Bio Lab Skills
Bradford Assay Quiz Questions
| Question | Answer |
|---|---|
| What is the primary purpose of the Bradford assay? | To determine the concentration of protein in a sample |
| Why does the absorbance of the Coomassie dye shift from 490 nm to 595 nm in the presence of protein? | Because protein binding changes the dye's charge and structure |
| Why is it recommended to perform assays in duplicate or triplicate? | To average out pipetting or measurement errors |
| If a student’s standard curve has the equation y = 0.012x + 0.05, and a sample’s absorbance (y) is 0.41, what is the sample’s protein concentration (x)? | 30 µg/mL |
| Why must we ensure equal enzyme concentrations when comparing mutant and wild-type KPR samples? | To ensure any rate differences are due to mutation, not protein amount |
| A student performing a Bradford assay combined 80µL of her sample with 80µL of water before adding dye. This gave an absorbance of 0.68. If the line of best fit is y = 0.014x + 0.03, what is concentration of her protein sample. | 92.8 µg/mL |
| You need 160 µL per well at 10 µg/mL from a 145 µg/mL stock, and you’re preparing 2 wells. How much stock is needed to make the 320 µL parental solution? | 22.1 µL |
Created by:
emily.zegarra