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Bio Lab Skills

PAGE Quiz Questions

QuestionAnswer
What chemical in the loading buffer provides density so the sample sinks into the well? Glycerol
Why are protein samples heated at 95°C before electrophoresis? denature proteins
What would happen if a student forgot to add beta-mercaptoethanol to the sample? Disulfide bonds would remain intact, so some proteins would not fully linearize.
A gel was allowed to run until the Bromophenol Blue band completely exited. What is the most likely outcome Some proteins may run out the gel and be lost.
What is the main difference between Native-PAGE and SDS-PAGE? Proteins remain folded and intact in Native-PAGE.
Which properties affect protein migration in Native-PAGE? charge density, size (#of Amino acids), and shape
Two proteins have identical molecular weights. In Native-PAGE, protein A is globular and protein B is elongated. Which migrates faster? protein A
Why is polyacrylamide gel used instead of agarose for protein separation? smaller pore size suitable for proteins
Which of the following is a limitation of Native-PAGE? cannot determine accurate molecular weight
A protein shows three bands in a Native-PAGE run, but only one band in SDS-PAGE. What does this suggest? protein exist in multiple oligomeric forms
You need to preserve enzyme activity while separating proteins by electrophoresis. Which approach is best? Native-PAGE without SDS
Created by: emily.zegarra
 

 



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