click below
click below
Normal Size Small Size show me how
ap bio unit 6a
| Question | Answer |
|---|---|
| topoisomerase: | thing on the very end of the dna to relieve tension to prevent dna breakage |
| SSBP: | binding proteins that stabilize open dna |
| RNA primase: | adds RNA nucelotides to dna so DNA poly 1 has double stranded area to attatch to |
| ligase uses what bonds? | covalent |
| silent mutation: | -change in 1 nucleotide -no change in AA and thus no change in protein function |
| missense mutation: | -change in 1 nucleotide -1 AA change and thus lose/dec function in protein |
| nonsense mutation: | -change in 1 nucleotide -AA now codes for a STOP protein, cutting the protein short too soon and thus is usually harmful |
| frameshift mutation: | -deletes or adds nucelotide 1 or more -results in different AA and maybe STOP codon = very harmful |
| hershey and chase experiments: | tags proteins with Sulfur and tags nucleic acids with Phosphorus that are added to bacteria, and found that nucelic acids's phosphorus is shown in the bacteria, proving that nucelic acids are contained with genetic material |
| promoter: | shows "start" (TATA box...) |
| transcription factors: | "bookmark" |
| enzyme that cuts introns: | spliceosome |
| codon chart: | dna --> mRNA (with uracil) --> read chart. *read the codon from the mRNA, NOT the tRNA!!!! |
| towards rep fork = | leading |
| away rep fork = | lagging |
| steps of PCR: | -denaturation: heat DNA to break H bonds and open it up -annealing: cool down, use a primer to show what u want to copy -extension: use dna polymerase to make copies of dna -repeat process 40 or more times!! |
| tag polymerase: | loves heat! allows u to heat dna to higher temps bc tag polymerase can withstand the heat :) |
| steps in protein synthesis: | -transcr - copy specifc gene NUCLEUS -mRNA procces - alternate splicing -translat - use mRNA code as directions to make AA chain/polypepti CYTOPLASM -post-translation (only in euk) - protein folding, add more stuff to make proteins new things CYTOPLASM |
| order of ribosomes transport: | rough ER --> vesicles for transport --> golgi --> membrane or export out of the cell or lysosome |
| acronym to help remmeber A, C, G, T pairings with pruines and pyrimidines: | -pure as gold -cut the pye :) |
| covalent vs H bonds on DNA: | covalent/phosphodiester: on the sides/sugar-phosphate backbone H bonds: in between the base pairs |
| meselson and stahl experiment: | -tagged dna/gave specific weight -determined that dna is semi-conservative |