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L1 Oxidase Test

L1 Oxidase Test - Tripticase Soy Agar (TSA)

QuestionAnswer
Name of this Test? Identify bacteria containing repiratory Enzyme CYTOCHROME-C for ELECTRON TRANSPORT. Ex: (+)Neisseria, (-)Entero-Bacteriaceae, (+)Pseudomonadaceae Oxidase Test
Oxidase Test: Name of MEDIUM? Trip-ticase Soy Agar Plate - NO CARBS!! - JUST AMINO ACIDS!! - COMPLEX type (don't know details of aminos)
Oxidase Test: Important INGREDIENTS in Medium? 1. Tripticase Soy Agar Plate: contains all AMINO ACIDS and no carbohydrates 2. BBL DrySlide: contains tetra-methyl-p-phenylene-diamine-di-hydrochloride as an INDICATOR (+) 20sec Dark blue/purple, (-) 20 sec no color change
Oxidase Test: How to INOCULATE? Streak Plate
Oxidase Test: How to INCUBATE Medium for Test? incubate at 35°C for 48 hours
Why must you test agains PSEUDOMONAS first in a OXIDASE TSA Test? 1. It's the CONTROL - Oxidase(+) 2. STANDARD TIME - time it takes to turn purple 3. Other bacteria must turn purple at this STANDARD TIME or is Oxidase(-)!!!
Whats another name for the Oxidase Test? Tripticase Soy Agar test
Whats another name for the Tripticase Soy Agar Test? Oxidase Test
What does TSA stand for? Tripticase Soy Agar
Created by: jviloria3
 

 



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