Not all of the host cells will sucessfully take up a recombinant plasmid 3 issues can occur

The recombinant plasmid doesnt get inside the cell The plasmid rejoins before the DNA fragment entered The DNA fragment stick to itslef rather than inserting into the plasmid

What do marker genes on the plasmid do

They can be used to identify which bacteria successfully took up the recombinant plasmid

What are the 3 Marker genes used

Antibiotic resistance genes Genes coding for fluorescent proteins Genes coding for enzymes

Antibiotic resistance marker genes

They are resistanant to tetracycline gene and Resistant to the ampicillin gene

Once DNA fragment is inserted

Resistance to the tetracycline gene is disrupted by DNA fragment This gene will no longer create a functional protein

Jellyfish contain a gene which codes to create a

Green fluorescent protein (GFP) This GFP gene can be inserted into the bacteria plasmid

When a Plasmid with a GFP gene in what happens when DNA fragment inserted

DNA fragment inserted in the middle of the GFP gene This disrupts it and prevents GFP production

The enzyme lactase can turn a certain substance blue from colourless

When a plasmid has a lactase gene in

The DNA fragment is inserted in the middle of the lactase gene. This disrupts it and prevents lactase production

What does a fermenter do

A fermenter is used to grow multiple copies of host cell which have been identified as containing the recombinant plasmid

This large cloned population of host cell

Can then produce protein coded for by the inserted DNA fragment

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Topic 8

Identifying transformed cells

Term	Definition
Not all of the host cells will sucessfully take up a recombinant plasmid 3 issues can occur	The recombinant plasmid doesnt get inside the cell The plasmid rejoins before the DNA fragment entered The DNA fragment stick to itslef rather than inserting into the plasmid
What do marker genes on the plasmid do	They can be used to identify which bacteria successfully took up the recombinant plasmid
What are the 3 Marker genes used	Antibiotic resistance genes Genes coding for fluorescent proteins Genes coding for enzymes
Antibiotic resistance marker genes	They are resistanant to tetracycline gene and Resistant to the ampicillin gene
Once DNA fragment is inserted	Resistance to the tetracycline gene is disrupted by DNA fragment This gene will no longer create a functional protein
	Grow bacterial on agar
	Transfer the bacterial colonies to a plate with ampicillin antibiotic in the agar
	Transfer the bacterial colonies to a plate with tetracycline antibiotic within the agar
Jellyfish contain a gene which codes to create a	Green fluorescent protein (GFP) This GFP gene can be inserted into the bacteria plasmid
When a Plasmid with a GFP gene in what happens when DNA fragment inserted	DNA fragment inserted in the middle of the GFP gene This disrupts it and prevents GFP production
Enzyme Markers	The enzyme lactase can turn a certain substance blue from colourless
	The gene for this enzyme is inserted into the plasmid The DNA fragment inserted in the middle of this gene to disrupt it The bacteria are then grown on an agar plate with the colourless substance
	The colonies which cannot turn the colourless substance blue contain the recombinant plasmid
When a plasmid has a lactase gene in	The DNA fragment is inserted in the middle of the lactase gene. This disrupts it and prevents lactase production
What does a fermenter do	A fermenter is used to grow multiple copies of host cell which have been identified as containing the recombinant plasmid
This large cloned population of host cell	Can then produce protein coded for by the inserted DNA fragment

Created by: yHya

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