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Topic 8

PCR in vitro clonign

TermDefinition
How can fragments of DNA be amplified This can be done in vitro via the polymerase chain reaction This is done by a automated machine
PCR equiptment list Thermocyler DNA fragment to be amplified DNA polymerase - taq polymerase Primers DNA nucleotides
PCR method step 1 The tempreature is first increased to 95 degree celcius which causes hydrogens bonds to break and DNA to split into single strands
step 2 The tempreature is then decreased to 55 so that primers can attach
step 3 The enzyme DNA polymerase then attacehs complemntary free nucleotides and makes a new strand align next to each template. The tempreature is increased to 72 degree celcius for this stage the optiumum for taq DNA polymerase
Advantages of PCR Automated- more efficient Rapid- 100 billion copies of DNA can be made within hours Doesnt require living cells- quicker and less complex techniques needed
Created by: yHya
 

 



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