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Bio 200 Unit 1

QuestionAnswer
fertilization sperm + egg = zygote
ploidy number of sets of chromosomes in nucleus
events of fertilization 1. recognition + contact between sperm and egg 2. regulation of sperm entry to prevent polyspermy 3. activation of quiescent egg 4. fusion of 2 haploid pronuclei from sperm + egg
gametes produces in gonads, produced by meiosis and cytokinesis
meiosis special type of division of nucleus, reduces chromosome number by 1/2
egg large, non-motile reproductive cell, fuses with sperm, provides nutrients + molecules to support early development of zygote into embryo
egg jell coat 3 key components - GAGs, sulfated polysaccharides, SAPS
GAGs large polysaccharides, attract and hold water, protect from dehydration
Sulfated polysaccharides in egg jelly coat, attract and activate sperm, may be species specific
SAPs in egg jelly coat, attract and activate sperm, may be species specific
cell membrane phospholipid bilayer, has pumps, channels, and receptors, highly specialized, microvilli facilitate sperm entry
vitelline layer fibrous glycoprotein mat, attached to cell membrane, receptor proteins on surface, facilitates sperm/egg binding via bindin protein receptors, forms protective fertilization envelope
cytoplasm contains all typical organelles, G-actin, microfilaments
G-actin protein, forms microfilaments, lots in egg cortex
microfilaments polymers of g-actin
egg cytoplasm contains specialized materials, yoke glycoproteins, morphogen, cortical vesicles
yoke glycoproteins provide nutrition to egg
morphogen molecules that direct cell specialization
cortical vesicles 15,000 per egg, below membrane, membrane bound sacs, contain GAGs, enzymes, hyaline
hyaline large adhesive protein
sperm small, motile reproductive cell, find and fuses with egg of same species, delivers haploid nucleus to egg
sperm head contains nucleus, acrosomal vesicle, pool of g-actin, cell membrane
acrosomal vesicle large membrane bound sac, contains bindin protein and enzymes that digest egg jelly
sperm midpiece contains mitochondria for energy, centrioles/microtubule organizing center
microtubule polymer of tubulin
sperm tail contains array of microtubules, sliding of Mts past each other leads to tail bending and swimming
chemotaxis movement in response to chemical gradient
acrosome reaction increase in calcium concentration triggers exocytosis of acrosomal vesicle to dump contents to outside, increase pH leads to polymerization of g-actin to form microfilaments
fertilization envelope slow, permanent block of polyspermy
cell cycle interphase, mitosis, cytokinesis
interphase majority of cell's life, stages - G1, S, G2
G1 interphase, basic metabolism and growth, prep for S phase
S phase interphase, synthesis of DNA in nucleus is replicated
G2 interphase, cell continues growing, prep for mitosis
mitosis division of genetic material - nucleus divides, resulting in 2 genetically identical daughter nuclei
cytokinesis division of cytoplasm, creates 2 genetically identical daughter cells
genetic material chromosomes, packed to fit in nucleus - chromatin and condensed chromosomes
chromatid relatively loose, thread-like, uncondensed, most of cell cycle
condensed chromosomes tightly folded, twisted configuration during mitosis
mitosis stages prophase, pro metaphase, metaphase, anaphase, telophase
prophase replicated chromosomes condense, mitotic spindle starts to form
prometaphase nuclear envelope breaks down, Mts of spindle bind to centromeres via protein disc-kinetochore, chromosomes pushed and pulled by spindle Mts
metaphase MTs have aligned chromosomes on midline, halfway between spindle poles
anaphase separase enzyme digests protein connections between sister chromatids, spindle Mts pull sister chromatids apart, each chromatid is a chromosome, Mts shorted to pull chromosomes to poles
telophase nuclear envelope forms, spindle disassembles, chromosomes decondense, 2 genetically identical nuclei
Created by: abby.c.chan
 

 



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