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Exam 1
Microbiology
| Term | Definition |
|---|---|
| Microbiology | The study of small biological specimens |
| Robert Hooke | (1635-1703) The first to describe microbes. Illustrated the fruiting structures of molds. |
| Antoni van Leeuwenhoek | (1632-1723) The first to describe bacteria. Further progress required development of more powerful microscope. Termed them Animalcules. One of the individuals referred to as the Father of Microbiology. |
| Louis Pasteur | (1822-1895) Discovered that living organisms discriminate between optical isomers. Disproved theory of spontaneous generation. Developed vaccines for anthrax, fowl cholera, and rabies. Invented the process of pasteurization. |
| Spontaneous Generation | Observation: Raw meat rots when left out. Theories: 1. Microorganisms arose spontaneously. 2. Microorganisms developed from seeds or germs from the air. |
| Francesco Redi | 1668 - disproves spontaneous generation. |
| John Needham | 1745 - Flask is boiled, flask remains open while cooling, flask is capped, microbial growth. Likely Explanations for result: 1. Did not boil the broth long enough to sterilize. 2. Possible contamination. 3. Possible presence of a bacterial endospore. |
| Lazzaro Spallanzani | 1768 - Experiment was conflicted due to "vital force" was left out (sealed flask). |
| Louis Pasteur (1) | 1859 - Swan Neck Flask Experiment: non sterile liquid poured into flask, neck of flask drawn out in flame, liquid sterilized by extensive heating. Liquid cooled slowly, liquid remains sterile indefinitely. |
| Louis Pasteur (2) | Flask tipped such that microorganism-laden dust contacts sterile liquid. Liquid putrefies. |
| Pasteurization | A process to decrease the number of microbes in a sample. This process is NOT sterilization. |
| Sterilization | The complete elimination of all living things. |
| Louis Pasteur's Vaccines | Fowl Cholerae, Anthrax Vaccine, Rabies Vaccine |
| Edward Jenner | 1796 - Pioneer of Vaccinology. Cowpox vaccine |