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Bio. U9 P1 Yr. 1
Bio. U9 Yr. 1
| Question | Answer |
|---|---|
| What non-coding regions are cut out of mRNA by the spliceosome and discarded before mature mRNA leaves the nucleus? | Introns |
| What strand of DNA is used as the template for synthesizing mRNA? | Anti-sense |
| A particular protein has a polypeptide chain that consists of 180 amino acids. What is the minimum number of nucleotides needed to code for this protein? | 540 |
| Replication, transcription, and translation all... | involve complementary base pairing |
| A change in a single base in DNA is called a... | point mutation |
| RNA editing, alternative splicing, and the addition of a 5' cap and poly-A tail all occur... | post-transcription |
| What is true regarding gene expression? | The genes that are transcribed depend on the function of the specific cell & a gene is a length of DNA that codes for a particular protein |
| Start codon is... | the three base sequence on mRNA that codes for Met (methionine) to be brought and translation to be initiated |
| Stop codon is... | the three base sequence on mRNA that signals the arrival of a release factor |
| E site is... | the site on the ribosome where tRNA detaches after its amino acid has been added to the polypeptide |
| P site is... | the binding site on the ribosome where a growing polypeptide strand is held and peptide bonds are actively formed |
| A site is... | the binding site on the ribosome where a new tRNA arrives carrying an amino acid for the growing polypeptide |
| Ribosome is an... | organelle that is the location of protein synthesis, found either "free" or bound to RER |
| What describes transcription? | Occurs in the nucleus of eukaryotes, adenine on DNA is paired with uracil on RNA, RNA polymerase is used, and uses DNA as a template to make mRNA |
| In eukaryotic cells, before pre-mRNA is mature mRNA that is ready to leave the nucleus, it needs to be edited. What modifications will take place? | A poly-A tail is added to the 3' end, alternative splicing may occur that cuts out specific exons for different variations of a protein to be made, introns are cut out and exons are spliced back together, a cap is added to the 5' end |
| A DNA triplet on the template strand that is transcribed has the bases TAG. What anticodon on tRNA is used in translation? | UAG |
| What is the correct sequence of events for recycling amino acids? | Protein tagged with ubiquitin --> protein is hydrolyzed by proteasome --> free amino acids/short polypeptides are released |
| What feature of the genetic code allows for human insulin to be produced by a bacterial cell? | universality |
| In transcription, what enzyme has a role similar to that of helicase in replication? | RNA polymerase |
| What role(s) does RNA polymerase play in transcription? | Adds new RNA nucleotides & unwinds DNA and separates the two strands |
| True or False? A change in a single DNA base can result in a non-functional protein. | True |