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Bio. U8 Yr. 1
| Question | Answer |
|---|---|
| What subunits would be connected by hydrogen bonds in DNA? | Cytosine to guanine |
| GelElectrophoresisIsAMethodOfSeparating_SegmentsByTheir_.ThePhosphateGroupsInThe_OfDNAGiveItA_Charge.Therefore,AfterPlacedInTheGel,TheDNAFragmentsMigrateTowardThe_End._FragmentsTravelFarther,ResultingInDistinctBandsThatAreAnIndividual's DNA "fingerprint." | DNA; size; backbone; negative; positive; smaller |
| A section of DNA contains 50 cytosine bases representing 10% of the total number of bases in this section. How many sugars and phosphate groups does this section contain? | 500 sugars, 500 phosphates |
| Which strand of DNA contains Okazaki fragments during replication? | Lagging strand |
| The phosphate group of one strand of DNA is called the ____ end. The free sugar end of the same strand is called the ____ end. | 5'; 3' |
| A particular sequence of DNA is found to be AGG-CAG-TTA. What would be the sequence of bases on the complementary strand of DNA? | TCC-GTC-AAT |
| Function during DNA replication: Helicase | Breaks hydrogen bonds between nitrogenous bases |
| Function during DNA replication: DNA Polymerase III | Adds new nucleotides to the 3' end |
| Function during DNA replication: DNA Polymerase | Removes RNA primers and replaces them with DNA nucleotides |
| Function during DNA replication: DNA Gyrase / Topoisomerase | Prevents supercoiling of DNA at the replication forks |
| Function during DNA replication: Primase | Produces short sequences of RNA which allows for the addition of DNA nucleotides |
| Function during DNA replication: SSB's | Keeps the single strands of DNA from re-annealing |
| A DNA molecule contains 21% cytosine. What percentage of adenine does this molecule contain? | 29% |
| What property of DNA did Rosalind Franklin's X-ray crystallography images show? | DNA is a double helix |
| How did Hershey and Chase's experiment provide evidence that DNA was the genetic material? | When bacteriophages were labeled with Phosphorus-32, bacteria became radioactive because the virus's DNA was inserted |
| Which property of DNA explains how genetic information can be replicated accurately? | The complementary base pairing |
| New strands of DNA are synthesized in which direction? | 5" —> 3' |
| Occurrence in the temperature cycle of the polymerase chain reaction (PCR): 1. Heating to 94oC | Denaturation / separating the two strands of DNA |
| Occurrence in the temperature cycle of the polymerase chain reaction (PCR): 2. Cooling to 55oC | Annealing / allowing primers to bind to the complementary DNA |
| Occurrence in the temperature cycle of the polymerase chain reaction (PCR): 3. Heating to 72oC | Elongation / allowing Taq polymerase to synthesize new DNA strands |
| What components make up a nucleotide of DNA? | Deoxyribose sugar, nitrogenous base, and phosphate group |
| The experiments of Meselson and Stahl provided evidence for... | The semi-conservative replication of DNA |
| DNA wraps around what proteins? | Histones |
| What is true regarding the polymerase chain reaction (PCR)? | PrePreparedPrimersDetermineRegionOfDNAToBeCopied,TemperatureIncreasedToDenature(Separate)TwoStrandsOfDNA,ATaqPolymeraseFromAThermophilicBacteriaIsUsedBecauseItCanWorkAtHigherTemperaturesThanOtherPolymerases,AndManyCopiesOfDNACanBeProducedInJustAFewHours |
| What enzyme is used in the polymerase chain reaction (PCR)? | Taq polymerase |
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user-1907392