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Fors bio- final

QuestionAnswer
DNA location mainly in the nucleus
Hair root Nuclear DNA
Hair shaft mitochondrial DNA
DNA in blood White blood cells only
Dye blobs unincorporated dye molecules, large peaks, appear in one color channel only
stutter peaks PCR slippage causes the polymerase to repeat or delete a repeat unit, typically one repeat shorter then the true allele.
incomplete adenylation taq polymerase may add an extra adenine results in two peaks one with an A addition and one without
Pull up/bleed-through fluorescence from one dye channel is detected in another. Usually appears at the same base pair size across channels.
spikes sharp peaks caused by electrical noise or capillary issues. narrow extremely tall and do not resemble allele peaks
 

 



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