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Bio 141 Unit 1
| Question | Answer |
|---|---|
| Location of translation in Eukaryotic and Prokaryotic Cells? | Euk: Nucleus Pro: Cytoplasm |
| Location of transcription in Eukaryotic and Prokaryotic Cells? | Cytoplasm in both |
| 3 types of RNA processing | 1. Capping 2.Add Poly A tail 3.Splicing |
| Where does each step of RNA processing occur? | Capping occurs in the 5' Adding a poly a tail occurs in the 3' end Splicing occurs throughout |
| What happens in each phase of RNA Processing? | Capping protects mRNA and is used for translation initiation Adding poly a tail protect mRNA and is used for transport/as a binding site Splicing removes introns/joins exons |
| How are mRNA sequences read by the translation machinery? | By Ribosomes (A-site, P-site, and E-site) and tRNA which bring in amino acids (Anticodon) |
| What is a codon? | A sequence of three nucleotides that code for specific amino acids |
| What is the open reading frame and how is it determined? | Sequence of DNA or RNA that can be translated into a protein, starting with a start codon and extending without interruption by a stop codon. |
| Ribosome A-site | Bind to arriving tRNA that carry Amino Acids |
| Ribosome P-site | Bind to tRNA carrying amino acids that formed peptide bonds |
| Ribosome E-site | Release/exit tRNAs that no longer carry amino acids |
| What is the function of tRNA? | connects mRNA to codons to the specific amino acids they encode |
| What determines where translation for a particular mRNA will begin | Start codon (AUG) |
| What is a mutation? What are the two main types? | changes in mRNA sequence. Point or Frameshift |
| Types of Frame shift | Insertion deletion |
| Types of point mutations | Silent: has no effect (codes for same amino acid) Missense: Changes sequence which changes function Nonsense: codes for stop codon |
| What is the chemical difference between ribose and deoxyribose? | Ribose has an OH atom at its 2' |
| Why is RNA less stable than DNA. | DNA contains deoxyribose which unlike ribose lacks oxygen the second carbon making it more stable. |
| What defines the 5' end of DNA | phosphate-bearing end |
| What defines the 3' end of DNA | hydroxyl-bearing end |
| What bond links sugar of one nucleotide with phosphate of another | phosphodiester bond |
| what bonds hold base pairs | hydrogen bond |