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Bio 141 Unit 1

QuestionAnswer
Location of translation in Eukaryotic and Prokaryotic Cells? Euk: Nucleus Pro: Cytoplasm
Location of transcription in Eukaryotic and Prokaryotic Cells? Cytoplasm in both
3 types of RNA processing 1. Capping 2.Add Poly A tail 3.Splicing
Where does each step of RNA processing occur? Capping occurs in the 5' Adding a poly a tail occurs in the 3' end Splicing occurs throughout
What happens in each phase of RNA Processing? Capping protects mRNA and is used for translation initiation Adding poly a tail protect mRNA and is used for transport/as a binding site Splicing removes introns/joins exons
How are mRNA sequences read by the translation machinery? By Ribosomes (A-site, P-site, and E-site) and tRNA which bring in amino acids (Anticodon)
What is a codon? A sequence of three nucleotides that code for specific amino acids
What is the open reading frame and how is it determined? Sequence of DNA or RNA that can be translated into a protein, starting with a start codon and extending without interruption by a stop codon.
Ribosome A-site Bind to arriving tRNA that carry Amino Acids
Ribosome P-site Bind to tRNA carrying amino acids that formed peptide bonds
Ribosome E-site Release/exit tRNAs that no longer carry amino acids
What is the function of tRNA? connects mRNA to codons to the specific amino acids they encode
What determines where translation for a particular mRNA will begin Start codon (AUG)
What is a mutation? What are the two main types? changes in mRNA sequence. Point or Frameshift
Types of Frame shift Insertion deletion
Types of point mutations Silent: has no effect (codes for same amino acid) Missense: Changes sequence which changes function Nonsense: codes for stop codon
What is the chemical difference between ribose and deoxyribose? Ribose has an OH atom at its 2'
Why is RNA less stable than DNA. DNA contains deoxyribose which unlike ribose lacks oxygen the second carbon making it more stable.
What defines the 5' end of DNA phosphate-bearing end
What defines the 3' end of DNA hydroxyl-bearing end
What bond links sugar of one nucleotide with phosphate of another phosphodiester bond
what bonds hold base pairs hydrogen bond
Created by: Star06
 

 



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