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| Term | Definition |
|---|---|
| State where the electron transport chain (ETC) occurs. | On the inner mitochondrial membrane (cristae). |
| Define oxidative phosphorylation. | Production of ATP using energy released by electron transport and chemiosmosis. |
| State the role of NADH and FADH₂ in the ETC. | They donate high-energy electrons to the electron transport chain. |
| Explain what happens to electrons as they move along the electron carriers. | Electrons move to lower energy levels, releasing energy stepwise. |
| State what the released energy from electrons is used for. | To pump protons (H+) from the matrix to the intermembrane space. |
| Describe the proton gradient. | A high concentration of H+ in the intermembrane space and low concentration in the matrix. |
| Define chemiosmosis. | Diffusion of protons through ATP synthase to generate ATP. |
| State the enzyme that synthesizes ATP. | ATP synthase. |
| Explain how ATP synthase works. | Protons flow down their gradient through ATP synthase, causing it to rotate and phosphorylate ADP to ATP. |
| State the final electron acceptor in aerobic respiration. | Oxygen. |
| Describe what oxygen becomes after accepting electrons. | Oxygen combines with electrons and protons to form water. |
| State why oxygen is essential for the ETC. | Without oxygen, electrons cannot be accepted, the chain stops, NADH cannot be oxidized, and ATP production halts. |
| Explain why the ETC produces the most ATP. | Most ATP is generated by chemiosmosis using the proton gradient made by electron transport. |
| State the approximate ATP yield from oxidative phosphorylation per glucose. | About 26–28 ATP. |
| Explain why mitochondria have many cristae. | Increased surface area allows more ETC complexes and ATP synthase → more ATP production. |
| Explain why FADH₂ produces less ATP than NADH. | FADH₂ donates electrons later in the chain, pumping fewer protons. |
| State the direction of proton pumping. | From the matrix to the intermembrane space. |
| Explain what happens if the inner mitochondrial membrane is damaged. | Proton gradient cannot form → ATP synthesis stops. |