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Cloning

Uni of Notts, Structure Function & Analysis of Genes, year 2, topic 11

TermDefinition
2 most common types of reverse transcriptase Moloney Murine Leukaemia virus (M-MuLV) derived & Avian Myeloid Leukaemia Virus (AMV) derived RT
Common reverse transcriptase modifications Enhanced thermostability, processivity, Ribonuclease Hybrid, & reduced probability of transcript truncation
Enzyme processivity An enzyme's ability to catalyse consecutive reactions without releasing the substrate (or just catalyses quickly)
Main synthetic RT variant (+ its statistics relative to others) SuperScriptIV. Enhanced thermostability (50*C optimum), x50 processivity
T4 DNA ligase function Uses rATP & Mg2+. Adenylates the 5' phosphate, very unstable leaving group making the phosphate an easy target for nucleophilic attack from the 3'-OH. Good for sticky & blunt ends
Alkaline phosphatase & benefits Dephosphorylates phosphate moieties at low pH & relatively low temperature leaving -OH groups in their place. They work well in the gut, reducing inflammatory signals & preventing demineralisation
Created by: Denny12
 

 



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