Unique challenges for DNA testing

need for high quality results every time Regulated environment care to prevent contamination politics and bureaucracy in govt labs

Challenges in presenting non-human DNA in court

novelty application (too new) validity of underlying scientific theory validity of statistical interpretations relevant scientific community to consult in the application is limited

Advantages of using DNA in mass disaster victim identification

SNPs are most common type of DNA sequence in the world HGP identified millions of variations and opened avenues of genetic analysis can be used on fragmented DNA

Disadvantages of using DNA in mass disaster victim identification

DNA degradation caused by environmental factors large number of samples Presence of mixed DNA expensive and slow

Additional uses of STR typing besides forensic DNA analysis

Ancestry DNA paternal and maternal testing mass disaster ID

Advantages of thermal stable, hot-start DNA polymerase

DNA will degrade during extensor of PCR and amplification wont happen without it

Effective means to prevent contamination

careful techniques, cleanliness, routine monitoring, keep pre and post PCR separately

Why are tetra-nucleotide repeat loci preferred over dinucleotide repeat loc in forensic testing

Dinucleotides are much more prone to producing stutter peaks in PCR Tetranucleotides give reliability and are able to be reproduced easily

Why is it valuable to include the sex-typing marker amelogenin in STR typing kit

Determines biological sex of the sample's owner. Amelogenin has different lengths on X and Y chromosomes and allows to determine M or F in criminal cases Serves as internal control, if amplification fails it means either poor DNA or PCR issues

What is electric-osmotic flow

negatively charged groups on surface of capillary and positively charged groups in buffer. creates flow of entire buffer

How does electric-osmotic flow impact DNA separation in capillary

DNA is negatively charged so it moves toward anode allows it to separate from anything combined into the DNA

What component of a PCR reaction is labeled with fluorescent dye to enable detection of amplified STR alleles

the primers used to amplify the DNA (forward or reverse specific-locus primer)

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Forensic Biochem 3

Question	Answer
Unique challenges for DNA testing	need for high quality results every time Regulated environment care to prevent contamination politics and bureaucracy in govt labs
Challenges in presenting non-human DNA in court	novelty application (too new) validity of underlying scientific theory validity of statistical interpretations relevant scientific community to consult in the application is limited
Advantages of using DNA in mass disaster victim identification	SNPs are most common type of DNA sequence in the world HGP identified millions of variations and opened avenues of genetic analysis can be used on fragmented DNA
Disadvantages of using DNA in mass disaster victim identification	DNA degradation caused by environmental factors large number of samples Presence of mixed DNA expensive and slow
Additional uses of STR typing besides forensic DNA analysis	Ancestry DNA paternal and maternal testing mass disaster ID
Advantages of thermal stable, hot-start DNA polymerase	DNA will degrade during extensor of PCR and amplification wont happen without it
Effective means to prevent contamination	careful techniques, cleanliness, routine monitoring, keep pre and post PCR separately
Why are tetra-nucleotide repeat loci preferred over dinucleotide repeat loc in forensic testing	Dinucleotides are much more prone to producing stutter peaks in PCR Tetranucleotides give reliability and are able to be reproduced easily
Why is it valuable to include the sex-typing marker amelogenin in STR typing kit	Determines biological sex of the sample's owner. Amelogenin has different lengths on X and Y chromosomes and allows to determine M or F in criminal cases Serves as internal control, if amplification fails it means either poor DNA or PCR issues
What is electric-osmotic flow	negatively charged groups on surface of capillary and positively charged groups in buffer. creates flow of entire buffer
How does electric-osmotic flow impact DNA separation in capillary	DNA is negatively charged so it moves toward anode allows it to separate from anything combined into the DNA
What component of a PCR reaction is labeled with fluorescent dye to enable detection of amplified STR alleles	the primers used to amplify the DNA (forward or reverse specific-locus primer)

Created by: Kayla_K

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