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UNIT 4, CHPT 13

CHAPTER 13.4

QuestionAnswer
what is nucleic acid hybridization? base pairing of one strand of a nucleic acid to a complementary sequence on a differnt nucleic acid strand
what is genetic engineering? direct manipulation of genes for practical purposes
what is DNA cloning? production of multiple copies of a specific DNA segment
what are plasmids? small circular DNA molecules that are replicated sepereately
what is a recombinant DNA molecule? molecule containing DNA from 2 different sources
what is gene cloning? production of multiple copies of a gene
what is a cloning vector? DNA molecule that can carry foreign DNA into a cell and be replicated there
what are restriction enzymes? endonuclease that recognizes and cuts DNA molecules foreign to a bacterium; cuts at specific nucletotide sequences
what is a restriction site? specific sequence on a DNA strand that is recognized and cut by a restriction enzyme
what are restriction fragments? DNA segment that results from the cutting of DNA by a restriction enzyme
what is gel electrophoresis? a technique which uses a gel made of polymer as a sieve to seperate a mixture of nucleic acid fragments by length
what is PCR? technique for amplifying DNA in vitro by incubating it with specific primers, a heat resistant DNA polymerase, and nucleotides
what is DNA sequencing? determines the order of nucletide bases in a gene or DNA fragment.
what is the CRISPR cas-9 system? technique for editting genes in living cells involving a bacterial protein called cas9 associated with a guide RNA complementary to a gene sequence of interest
what is a gene drive? a process that biases inheritance so that a particular allele is more likely to be inherited
Created by: SuperBugLover
 

 



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