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Genetics Terms
Genetics terms for Bio 121
| Term | Definition |
|---|---|
| restriction enzyme | an enzyme that cuts a DNA strand wherever it finds a specific short sequence of base pairs |
| gel electrophoresis | a method of separating macromolecules by size and charge by using electrical attraction to pull the molecules through a porous gel |
| PCR | Polymerase chain reaction. A technique to amplify DNA by repeatedly cycling through rounds of denaturing DNA, annealing specific primers flanking the site to be amplified, and copying DNA with a polymerase. |
| CRISPR-Cas9 | A gene editing tool that allows DNA sequences to be altered in almost any way desired in a living cell. |
| transcription | The copying of a gene in a cell’s DNA into an RNA transcript. |
| intron | A sequence in a eukaryotic gene that is transcribed but will not be translated. Introns must be spliced out of the raw mRNA transcript before it leaves the nucleus to be translated. |
| Poly-A tail | A sequence of adenine (A) nucleotides added to the end of eukaryotic mRNA transcripts before they leave the nucleus for translation - length of the poly-A tail determines how many times the mRNA can be translated before it is degraded in the cytoplasm |
| degenerate code | A code in which there is not a one-to-one correspondence between code signals and their translations. |
| tRNA | A small RNA molecule that binds to a specific amino acid and to a specific codon in an mRNA molecule. tRNA molecules act as the means for the ribosomes to assemble a polypeptide with the correct amino acids specified in the mRNA code. |
| operon | A set of genes in a prokaryote that are adjacent to each other and that share a common promoter and regulatory mechanisms. |
| in-situ hybridization | A method for detecting gene expression in tissues by binding an RNA probe (labeled with a dye or fluorescent marker) to mRNA in a fixed tissue sample. It allows detection of gene expression in a specific tissue at a specific point in time. |
| translation | The reading of an mRNA code by a ribosome and tRNA molecules and the resulting construction of a polypeptide based on that code. |