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Bio Final

QuestionAnswer
genetic recombination term for the production of offspring with combos of traits that differ from those found in either parent
genomic imprinting phenomenon in which expression of an allele in offspring depends on whether the allele is inherited from the male or female parent
quantitative character heritable feature that varies continuously over a range rather than in an either or fashion (white, pink, red)
laws of segregation Mendels 1st law stating that the 2 alleles in a pair segregate into different gametes during gamete formation
recombinant type (recombinant) offspring whose phenotype differs from that of the true-breeding P generation parents; also refers to the phenotype itself
heterozygote an organism that has 2 diff alleles for a gene
recessive allele an allele whose phenotypic effect is not observed in a heterozygote
hybridization mating/crossing of two true breeding varieties
dihybrid -heterozygous with respect to 2 genes of interest -all offspring from a cross between homozygous parents are dihybrids
linked genes genes located close together on the same chromosome
heterozygous 2 diff alleles for a given gene
sex-linked gene -gene located on either sex chromosome -Most located on X chromosome
chromosome theory of inheritance genes are located at specific position on chromosomes and the behavior of chromosomes during meiosis accounts for inheritance patterns
incomplete dominance situation in which the phenotype of heterozygotes is intermediate between the phenotypes of individuals homozygous for either allele
character observable heritable feature that may vary among individuals
allele any alternative version of a gene that may produce distinguishable phenotypic effects
translocation -aberration in chromosome structure resulting form attachment of a chromosomal fragment to a nonhomologous chromosome
wild type phenotype most commonly observed in natural populations
X-linked gene gene located on X chromosome
dominanat allele an allele that is fully expressed in the phenotype of a heterozygote
pedigree -diagram of family tree with conventional symbols showing the occurrence of heritable characters in parents and offspring over multiple gens
homozygote org has a pair of identical alleles for a gene
inversion aberration in chromosome structure resulting in reattachment of a chromosomal fragment in a reverse orientation to the chromosome from which it originated
duplication aberration in chromosome structure due to fusion with a fragment from a homologous chromosome such that a portion of a chromosome is duplicated
complete dominance phenotypes of a heterozygote and dominant homozygote are indistinguishable
homozygous 2 identical alleles for a given gene
polyploidy extra sets of chromsosomes
testcross breeding an org of unknown genotype with a homozygous recessive individual to determine the unknown genotype. The ratio of phenotypes in the offspring reveals the unknown genotype
nondisjunction error in mitosis or meiosis in which members of a pair of homologous chromosomes or a pari of sister chromatids fail to separate properly form each other
transcription synthesis of RNA using a DNA template
phenotype the observable physical and physiological traits of an organism which are determined by genetic makeup
genotype the genetic makeup or set of alleles of an org
law of independent assortment -Mendels 2nd law -each pair of alleles segregates or assorts independently of each other pair during gamete formation -applies when genes are not super close together
parental type offspring with a phenotype that mates one of the true breeding parental phenotypes
carrier individual who is heterozygous at a given genetic locus for a recessive disorder
addition rule rule of probability stating that any one of two or more mutually exclusive events occurring can be determined by adding their individual probabilities
epistasis type of gene interaction in which the phenotypic expression of one gene alters that of another independently inherited gene
multiplication rule rule in probability stating that the probability of two or more independent events occurring together can be deterred by multiplying their individual probabilities
trait one of two or more detectable variants in a genetic character
ture-breeding orgs that produce offspring of the same variety over many gens of self pollination
codominanace phenotypes of both alleles are exhibited in the heterozygote because both alleles affect the phenotype in separate distinguishable ways
deletion -deficiency in a chromosome resulting from the loss of a fragment -mutual loss of one or more nucleotide pairs from a gene
P generation ture-breeding (homozygous) parents from which F1 hybrid offspring are derived in studies of inheritance
crossing over reciprocal exchange of genetic material between non sister chromatids during prophase 1 of meiosis
aneuploidy abnormal chromosome number
monohybrid -an org that is heterozygous with respect to a single gene of interest -all offspring from a cross between parents homozygous for diff alleles are monohybrids (AA+aa=Aa)
polygenic inheritance additive effect of two or more genes on a single phenotypic character
Features of sci inquiry -observation -question -research -hypothesis -experiment - analyze data -conclusion -communicate results
Inquiry the search for info and explanations of natural phenomena
euchromatin less condensed form of eukaryotic chromatin that is available for transcription
bacteriophage virus that infects bacteria (also called a phage)
telomere -tandemly repetitive DNA at the end of a eukaryotic chromosomes DNA molecule -protect the organisms genes from being eroded during successive rounds of replication
semiconservative model Type of DNA replication in which the replicated double helix consists of one old strand and one new strand
primer -short polynucleotide -bound by complementary base pairing to template strand -Elongated with DNA nucleotides during replication
chromatin complex of DNA and proteins that makes up eukaryotic chromosomes
helicase -enzyme that untwists the double helix of DNA at replication forks -separates the two strands making them available as template strands
origin of replication site where the replication of a DNA molecule begins. consisting of a specific sequence of nucleotides
topoisomerase -helps to relieve strain in the double helix ahead of the replication fork -brekas, swivels, and rejoins DNA strands
single-strand binding protein -protein that binds the unprepared DNA strands during DNA replication -stabilizes them and holds them apart while they serve as templates for synthesis of complementary strands of DNA
heterochromatin eukaryotic chromatin that remains highly compacted during interphase and is generally not transcribed
virus infectious particle incapable of replicating outside of a cell, consisting of RNA or DNA genome surrounded by a protein coat
primase enzyme that joins RNA nucleotides to make a primer during DNA replication (uses parental DNA strand as template)
nuclease enzyme that cuts DNA or RNA either removing one or a few bases or hydrolyzing the DNA or RNA completely into its component nucleotides
DNA replicaiton process by which a DNA molecule is copied
replication fork -Y shaped region on a replicating DNA moleu le where the parental strands are bing unwound and the new strands are being synthesized
DNA ligase -linking enzyme essential for DNA replication -catalyzes the covalent bonding of the 3' end of one DNA fragment to the 5' end of another
nucleotide excision repair repair system that removes then correctly replaces a damaged segment of DNA using the undamaged strand as a guide
mismatch repair cellular process the uses specific enzymes to remove and replace incorrectly paired nucleotides
DNA polymerase enzyme that catalyzes the elongation of new DNA by addition of nucleotides to the 3' end
nucleosome basic bead like unit of DNA packing in eukaryotes consisting of a segment of DNA wound around a protein core composed of two copies of each of the four types of histone
leading strand new complimentary DNA strand synthesized continuously along the template strand toward the replication fork in the mandatory 5 --> 3 direction
lagging strand discontinuously synthesized DNA strand that elongates by means of Okazaki fragments
histone small protein with a high proportion of positively charged amino acids that binds to the negatively charged DNA and plays a key role in chromatin structure
transformation -process by which a cell in culture acquires the ability to divide indefinitely -change in genotype and phenotype due to assimilation of external DNA by a cell
Okazaki fragment -short segment of DNA synthesized away from the replication fork on a template strand during replication -many of such segments are joined tougher to make up the lagging strand of newly synthesized DNA
double helix -form of native DNA -refers to its two adjacent antiparallel polynucleotide strands wound around an imaginary axis into a spiral shape
How do questions and research relate to biology allow you to explore and discover nature
community analysis and feedback -Peer review -Replicate findings -Publication -Consensus building
Social benefits and outcomes -Inform policy -Develop tech -solve problems -build knowledge
genetic code set of rules by which info encoded in genetic material is translated into proteins
flow of genetic code DNA strand (permanent) + Non template coding strand -->transcribed into mRNA --> translated into protein
transcription -gene determines sequence of nucleotide bases along the length of RNA -RNA synthesized in antiparallel direction to template strand of DNA
triplets of nucleotide bases -Smallest units of uniform length the can code for amino acids -64 possible code words -flow of info based on triplet code
Triplet code -transcribed into complementary series of 3 nucleotide words in m-RNA - translated into amino acid chain
Translation -sequence of codons along mRNA decoded into a segment of amino acids -each codon specifies which 1 of 20 amino acids will incorporate at corresponding position along polypeptide
How was genetic code determined -Series of experiments in the 60's disclosed amino acid translations of each mRNA codon -Nierenberg ('61) synthesized mRNA of only Uracil which translated into phenylalanine (F) -A,C, G similarly identified
genetic engineering -direct manipulation of genes for practical purposes -allow for researchers to exploit principle of complimentary base pairing to determine complete nucleotide sequence of DNA
1st automated genetic engineering -60's -Sanger -dideoxy sequencing (first automated procedure)
"next gen sequencing" techniques -Early 2000's -rapid and less expensive -single template strand immobilized and complimentary strand synthesized
3rd gen sequencing -Even faster and less expensive -single long dan sequence on its own -move DNA through small pore and ID bases one by one -Nanopore sequencer
Nanopore sequencer -2015 -size of candy bar; connects to computer via USB -immediate ID and analysis of DNA sequence
DNA cloning -isolates segment of DNA and makes copies -requires restriction enzymes
restriction enzymes -60's -cut dna in certain locations
Polymerase chain reactions -key to automation for genetic engineering -only needs small amount of DNA -used to provide DNA for cloning
what is the start codon for replicating DNA AUG - Methenamine
gene expression process by which information encoded in DNA directs the synthesis of proteins
mRNA -synthisized using DNA template -attaches to ribosomes in cytoplasm and specifies the primary structure of protein
spliceosome large complex made up of proteins and RNA molecules that splices RNA by interacting with ends of an RNA intron releasing the intron and joining the two adjacent exons
silent mutation -nucleotide-pair substitution that has no observable effect on the phenotype -ex. a mutation that results in a codon that codes for the same amino acid
transcription initiation complex completed assembly of transcription factors and RNA polymerase bound to a promoter
gene editing altering genes in a specific, predictable way
start point transcription - the nucleotide position on the promoter where eRNA polymerase begins synthesis of RNA
exon -sequence within a primary transcript that remains in the RNA after RNA processing -also referes to the region of DNA from which this sequence was transcribed
5' cap modified form of guanine nucleotide added onto the 5' end of a pre-mRNA molecule
deletion -deficiency in a chromosome resulting from the loss of a fragment though breakage
aminoacyl-tRNA synthetase enzyme that joins each amino acid to the appropriate tRNA
RNA processing modification of RNA primary transcripts, including splicing out of introns, joining together of exons, and alteration of the 5' and 3' ends
promoter specific nucleotide sequence in the DNA of a gene that binds RNA polymerase, positioning it to start transcribing RNA at the appropriate place
mutation a change in the nucleotide sequence of an orgs DNA or in the dna or rna of a virus
alternative RNA splicing a type of eukaryotic gene regulation at the rna processing level in which different mRNA molecules are produced froth same primary transcript, depending on which RNA segments are treated as exons and which as introns
transcription factor regulatory protein that binds to DNA and affects transcription of specific genes
point mutation change in a single nucleotide pair of a gene
ribosome complex of rRNA and protein molecules that functions as a site of protein synthesis inn the cytoplasm
nucleotide-pair substitution type of point mutation in which one nucleotide in a DNA strand and its partner in the complementary strand are replaced by another pair of nucleotides
transcriiption synthesis of RNA using a DNA template
translation -synthesis of a polypeptide using the genetic info encoded in an mRNA molecule -change of "language" from nucleotides to amino acids
P site one of a ribosomes three binding sites for tRNA during translation. It holds tRNA carrying the growing polypeptide chain
RNA splicing removal of portions of the transcript (introns) that will not be included in mRNA and the joining together of the remaining portions (exons)
ribosomal RNA (rRNA) RNA molecules that make up ribosomes
coding strand contemplate strand of DNA which has the same sequence as the mRNA except it has Thymine instead of Uracil
TATA box DNA sequence in eukaryotic promoters crucial in forming the transcription initiation complex
reading frame On a mRNA the triplet grouping of ribonucleotides used by the translation machinery during polypeptide synthesis
transcription unit region of DNA that is transcribed into an RNA molecule
template strand DNA strand that provides the pattern or template for ordering by complementary base pairing the sequence of nucleotides in an RNA transcript
triplet code genetic info system in which a series of three nucleotide long words specifies a sequence of amino acids for a polypeptide chain
insertion a mutation involving the addition of one or more nucleotide pairs to a gene
domain -taxonomic category above the kingdom level. -discrete structural and functional region of a protein
intron noncoding, intervening sequence within a primary transcript that is removed from the transcript during RNA processing; also the region of DNA form which the sequence was transcribed
polyribosome group of several ribosomes attached to, and translating the same messenger RNA molecule
codon 3 nucleotide sequence of DNA or mRNA that specifies a particular amino acid or termination signal; basic unit of genetic code
ribozyme An RNA molecule that functions as an enzyme, such as an intron that catalyzes its own removal during RNA splicing
Transfer RNA (tRNA) RNA molecule that functions as a translator between nucleic acid and protein languages by picking up a specific amino acid and carrying it to the ribosome, where tRNA recognizes the appropriate codon in the mRNA
primary transcript Initial RNA transcript from any gene; also called pre-mRNA when transcribed from a protein coding gene
mutangen chemical or physical agent that interacts with DNA and can cause a mutation
nonsense mutation mutation that changes an amino acid codon to one of the 3 stop codons, resulting in a shorter and usually nonfunctional protein
terminator in bacteria a sequence of nucleotides in DNA that marks the end of a gene and signals RNA polymerase to release the newly made RNA molecule and detach from the DNA
RNA polymerase enzyme that links ribonucleotides into a growing RNA chain during transcription, based on complementary binding to nucleotides on a DNA template strand
frameshift mutation mutation occurring when nucleotides are inserted in or deleted from a gene and the number inserted or deleted is not a multiple of 3, resulting in the improper grouping of the subsequent nucleotides into codons
anticodon nucleotide triplet at one end of a tRNA molecule that base pairs with a particular complementary codon on an mRNA molecule
signal peptide sequence of about 20 amino acids at or near the leading end of a polypeptide that targets it to the endoplasmic reticulum or other organelles in a eukaryotic cell
cloning vector -DNA molecule that can carry foreign DNA into a host cell and replicate there
recombinant DNA molecule DNA molecule made in vitro with segments form different sources
tumor suppressor gene gene whose protein product inhibits cell division thereby preventing the uncontrolled cell growth that contributes to cancer
genomics systematic study of whole sets of genes and their interactions
in vitro mutagenesis technique used to discover the function of a gene by cloning it, introducing specific changes, reinserting the stated gene and studying the phenotype
maternal effect gene a gene that, when mutant in the mother, results in a mutant phenotype in offspring
histone acetylation attachment of acetylene groups to certain amino acids of histone proteins
genetic engineering direct manipulation of genes for practical purposes
determination progressive restriction of developmental potential in which the possible fate of each cell becomes more limited as an embryo develops
nucleic acid probe -used to locate specific nucleotide sequences in a nucleic acid sample -molecules hydrogen bond to complementary sequence
alternative RNA splicing type of eukaryotic gene reg at the rna processing level in which different mRNA molecules are produced from the same primary transcript depending on which RNA segments are treated as exons and introns
genetic profile individuals unique set of genetic markers
homeotic gene -any of the master regulatory genes -control placement and spatial org of body parts in animals, plants, fungi by controlling developmental fate of group cells
RNA interface (RNAi) -mechanism for silencing expression of specific genes -RNA molecules are processed into siRNAs that either block translation or trigger degradation of the genes mRNA
operator -sequence of nucleotides near the start of an operon to which an active repressor can attach -binding of the repressor prevents RNA polymerase from attaching to the promoter and transcribing genes of the operon
DNA cloning production of multiple copies of a specific DNA segment
differential gene expression expression of different sets of genes by cells with the same genome
short tandem repeat (STR) -simple sequence DNA containing multiple tandemly repeated units of two to five nucleotides -Variations act as genetic markers in STR analysis used to prepare genetic profiles
plasmid -small, circular double stranded DNA molecule that carries accessory genes separate from those of a bacterial chromosome -used as vectors in DNA cloning
Human genome project an international collaborative effort to map and sequence the DNA of the human genome
repressor -protein that inhibits gene transcription
pseudogene -DNA segment that is very similar to a real gene, but does not yield a functional product -inactivated due to a mutation
transgene gene that has been transferred naturally or by a genetic engineering technique from one organism to another
sticky end single stranded end of a double stranded restriction fragment
transposon transposable element that moves within a genome by means of a DNA intermediate
small interfering RNA (siRNA) -one of multiple small, single stranded, RNA molecules generated by cellular machinery from a double stranded RNA molecule -associate with one or more proteins in a complex that can degrade or prevent translation of an mRNA with a complementary sequence
restriction enzyme -endonuclease that recognizes and cuts DNA molecules foreign to a bacterium -cuts at specific nucleotide sequences
long noncoding RNA (IncRNA) -RNA between 200 and hundreds of thousands of nucleotides n length that does not code for protein but is expressed at significant levels
polymerase chain reaction (PCR) technique for amplifying DNA in vitro by incubating ti with specific primers, a heat resistant DNA polymerase, and nucleotides
inducer specific small molecule that binds to a bacterial repressor protein and changes the repressors shape so that it cannot bind to an operator thus switching an operon on
regulatory gene gene that codes for a protein, such as a repressor, that controls the transcription of another gene or group of genes
morphogen a substance that provides positional information in the form of a concentration gradient along an embryonic axis
nucleic acid hybridization base pairing of one strand of a nucleic acid to the complementary sequence on a stand from another nucleic acid molecule
gene drive process that biases inheritance such that a particular allele is more likely to be inherited than are other alleles causing the favored allele to spread through the population
oncogene gene found in viral or cellular genomes that is involved in triggering molecular events that can lead to cancer
DNA sequencing determining the complete nucleotide sequence of a gene or DNA segment
pattern formation development of a multicellular organisms spatial organization the arrangement of organs and tissues in their characteristic places in 3D space
totipotent describing a cell that can give rise to all parts of the embryo and adult, as well as extraembryonic membranes in species that have them
single nucleotide polymorphism (SNP) single base pari site in a genome where nucleotide variation is found in at least 1% of the pop
epigenetics study of the inheritance of traits transmitted by mechanisms that do not involve the nucleotide sequence
RNA sequencing method of analyzing large sets of RNAs that involves making cDNAs and sequencing them
transposable element segment of DNA that can move within the genome of a cell by means of DNA or RNA intermediate
control element segment of noncoding DNA that helps regulate transcription of a gene by serving as a binding site for a transcripition factor
differentiation the process by which a cell or group of cells becomes specialized in structure and function
gene cloning production of multiple copies of a gene
complementary DNA (cDNA) -double stranded DNA molecule made in vitro using mRNA as a template and the enzymes reverse transcriptase and DNA polymerase
multigene family collection of genes with a similar or identical sequence
repetitive DNA -nucleotide sequences, usually noncoding, that are present in many copies in a eukaryotic genome
activator protein that binds to DNA and stimulates gene transcription
in situ hybridization technique using nucleic acid hybridization with a labeled probe to detect the location of a specific mRNA in an intact org
simple sequence DNA DNA that contains many copies of tandemly repeated short sequences
ras gene -gene that codes fro RAS, a G protein that relays a growth signal from a growth factor receptor to the plasma membrane to a cascade of protein kinases -results in stimulation of the cell cycle
cytoplasmic determinant -maternal substance - when placed into an egg influcences the course of early development by regulating expressions of genes that affect developmental fate of cells
morphogenesis development of the form of an organism and its structures
pluripotent describing a cel that can give rise to many, but not all, parts of an organism
reverse transcriptase-polymerase chain reaction (RT-PCR) -technique for determining expression of a particular gene -uses reverse transcriptase and DNA polymerase to synthesize cDNA from all the mRNA in a sample -then subjects the cDNA to the PCR amplification using primers specific to the gene of interest
cyclic AMP (cAMP) common chemical signal that has a diversity of roles (messenger/regulator)
induction process in which a group of cells or tissues influences the development of another group through close-range interactions
retrotransposon transposable element that moves within a genome by means of RNA intermediate, a transcript of the retrotransposon DNA
stem cell -any relatively unspecialized cell that can produce two identical daughter cells or two more specialized daughter cells that can undergo further diffusion
DNA methylation -presence of methyl groups on DNA bases (usually cytosine)
restriction fragment DNA segment the results from cutting of DNA by a restriction enzyme
transgenic pertaining to an org whose genome contains DNA introduced from another org of the same or diff species
restriction site specific sequence of DNA strand that is recognized and cut by a restriction enzyme
reference genome complete sequence that researchers agree best represents the genome of a given species
metagenomics -collection + sequencing of DNA from a group of species -computer software sorts partial sequences and assembles them into genome sequences of individual species making up the sample
expression vector -cloning vector that contains highly active bacterial promoter just upstream of the reaction site -allows gene to be expressed in a bacterial cell
gene therapy intro of genes into an afflicted individual for therapeutic purposes
proteome entire set of proteins expressed by a given cell, tissue, or org
operon unit of genetic function found in bacteria and phages consisting of a promoter, operator, and coordinately regulated cluster of genes whose products function in a common pathway
Barr Body Dense object lying along the inside of the nuclear envelope in cells of female mammals, representing highly condensed, inactivated X chromosome
gel electrophoresis technique for separating nucleic acids or proteins on the basis of their size and electrical charge
codons hold genetic information
When Thomas Hunt Morgan crossed red eyed F-1 Generation flies to each other, the F2 Generation included both red nd white eyed flies, but all flies were male. Which best explains morgans result The gene involved the Y chromosome
The SRY gene is best described as a gene present on the Y chromosome
The chromosomal alteration that results from a chromosome fragment joined to a non homologous chromosome is called a translocation
which of the following statements regarding gene linkage is correct the closer the two genes are on a chromosome, the lower the probability that crossover will occur between them
If a pair of homologous chromosomes fails to separate during meiosis 1, select the choice that shows the number of chromosomes of the four resulting gametes with respect to normal haploid number (n) n+1, n+1, n-1, n-1
German scientist Carl Correns found that inheritance of variegated color on the leaves of certain plants was determined only by the maternal parent. which of the following best explains this pattern of inheritance inheritance of plastid genes
Griffith heat killed a culture of pathogenic bacteria. He split the sample and injected half of it into mice. The mice lived. He then mixed the other half with a living, nonpathogenic bacteria strain and injected the mixture into mice. The mice died. A substance had been transferred from pathogenic to nonpathogenic
Which of the following statements describes the process of transformation in bacteria external DNA is taken into account cell becoming part of the cells genome
which of the following characteristics allowed hershey and chase to determine whether the genetic material was made of DNA or protein DNA contains phosphorous whereas protein does not
Which of the following statements accurately describes differences between DNA replication in prokaryotes and DNA replication in eukaryotes prokaryotic chromosomes have single origin of replication, whereas eukaryotic chromosomes have many
radish flowers may be red, purple, or white. A cross between red and white yields all purple offspring. What inheritance pattern is this? incomplete dominance
catabolism breaks down complete molecules releasing energy
cellular respiration catabolic pathway of aerobic and anaerobic respiration which breaks down organic molecules and uses electron transport chain to produce ATP
Glycolysis glucose split into pyruvate yielding a small amount of ATP
citric acid cycle further breaks down pyruvate to price more ATP and electron carriers
electron transport chain uses electron carriers to generate large amount of ATP
Anabolism builds complex molecules from simpler ones, requires energy
Photosynthesis conversion of light nrg to chemical nrg that is stored in sugar and other organic compuntds (Creates glucose from CO2 and H2O)
similarities in major metabolic pathways between plants and animals both use cellular respiration to convert energy in organic molecules like ATP (catabolic pathways)
differences in major metabolic pathways between plants and animals plants preform photosynthesis in chloroplasts to capture light and build energy
mitosis -process of nuclear division in eukaryotic cells conventionally divided into 5 stages -Conserves chromosome # by allocating replicated chromosomes equally to each daughter nuclei
meiosis -modified type of cell division in sexually reproducing orgs consisting of two rounds of cell division but only one round DNA relocation -Results in cells with half the number of chromosome sets
mitosis mechanism one round of division following DNA replication resulting in 2 genetically identical diploid daughter cellls -Pro, Prom, Meta, Ana, Telo
Prophase chromatin condenses into discrete chromosomes, mitotic spindle beings to form, nucleolus disappears
Prometaphase nuclear envelope fragments and spindle microtubules attach to kinetochores of chromsomes
Metaphase individual chromosomes line up at cells equator
anaphase sister chromatids separate and move to opposite poles
telophase nuclear envelopes reform and cell divides
Prophase 1 homologous chromosomes pari up and exchange genetic material
metaphase 1 homologous chromosomes line up at middle
anaphase 1 homologous chromosomes separate and move to opposite sides
telophase 1 two haploids form
Created by: bouzianeju
 

 



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