Lactose fermentation test using phenol red lactose broth red (PR lactose broth)

Yellow and red liquid tube with little tube inside

Lactose fermentation test using phenol red lactose red (PR lactose broth) E.coli Lactose fermentation and gas

positive yellow for lactose fermentation positive for gas bubble in little tube

Lactose fermentation test using phenol red lactose red (PR lactose broth) Salmonella typhimurium fermentation and gas

negative red for lactose fermentation negative for gas production no bubble

Lactose fermentation test using phenol red lactose broth red (PR lactose broth)test 3 compoments of this test

1. Lactose 2. Phenol Red 3. Durham Tube

Lactose fermentation test using phenol red lactose broth red (PR lactose broth)Ph indicator for this test

Alkakine PH - cerise pink Neutral pH - red Acidic pH- yellow

Mac/EMB/ HEA test for

Bile salt/ dye tolerance and lactose fermentation HEA test for H2S as well

Positive for bile salt/ dye tolerance Positive for lactose fermentation (pink)

Mac salmonella typhimurium

positive for bile salt and dye tolerance negative for lactose fermentation (tan)

positive for bile salt/ dye tolerance postive for lactose fermentation ( metalic shien )

EMB salmonella typhimurium

positive for bile sALT/ DYE TOLERANCE negative for lactose fermentation transparent color

Postive bile salt/dye tolerance positive for lactose fermentation (orange) postive for H2s (orange)

HEA salmonella typhimurium

positive for bile salt/ dye tolerance negative for lactose fermentation (green) positive for H2S black

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ASSessment BIo 150

Question	Answer
Lactose fermentation test using phenol red lactose broth red (PR lactose broth)	Yellow and red liquid tube with little tube inside
Lactose fermentation test using phenol red lactose red (PR lactose broth) E.coli Lactose fermentation and gas	positive yellow for lactose fermentation positive for gas bubble in little tube
Lactose fermentation test using phenol red lactose red (PR lactose broth) Salmonella typhimurium fermentation and gas	negative red for lactose fermentation negative for gas production no bubble
Lactose fermentation test using phenol red lactose broth red (PR lactose broth)test 3 compoments of this test	1. Lactose 2. Phenol Red 3. Durham Tube
Lactose fermentation test using phenol red lactose broth red (PR lactose broth)Ph indicator for this test	Alkakine PH - cerise pink Neutral pH - red Acidic pH- yellow
Lactose fermentation test using phenol red lactose broth red (PR lactose broth)how is gas detected for this test	A bubble in the durham tube
MAC	MacConkey agar
EMB	Eosin Methylene Blue agar
HEA	Hektoen Enteric Agar
Mac/EMB/ HEA test for	Bile salt/ dye tolerance and lactose fermentation HEA test for H2S as well
Mac E coli	Positive for bile salt/ dye tolerance Positive for lactose fermentation (pink)
Mac salmonella typhimurium	positive for bile salt and dye tolerance negative for lactose fermentation (tan)
EMB E. coli	positive for bile salt/ dye tolerance postive for lactose fermentation ( metalic shien )
EMB salmonella typhimurium	positive for bile sALT/ DYE TOLERANCE negative for lactose fermentation transparent color
HEA E.coli	Postive bile salt/dye tolerance positive for lactose fermentation (orange) postive for H2s (orange)
HEA salmonella typhimurium	positive for bile salt/ dye tolerance negative for lactose fermentation (green) positive for H2S black

Created by: alejac0360

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