Help
Options
Didn't know it?
click below
click below
Knew it?
click below
click below
Don't Know
Remaining cards (0)
Know
retry
shuffle
restart
0:00
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
bio162 e3:G1 Checkpt
| Question | Answer |
|---|---|
| Kinase | Adds a phosphate |
| cyclins and CDKs | the "cell cycle check" system |
| Transcription Factors | required to turn on a gene |
| p16 | A CDK inhibitor; specifically, it's a competitive inhibitor of CDK 4/6 |
| Cyclin D | protein coenzyme for CDK 4/6 |
| CDK 4/6 | the CDK that binds to cyclin D |
| Rb protein | Transcription factor inhibitor |
| TFE2F | transcription factor for cyclin E |
| Cyclin E | the protein for CDK 2 that allows transition from G1 to S |
| CDK 2 | the CDK that binds to cyclin E and cyclin A |
| p27 | another CDK inhibitor (like p16) that is targeted by the CDK 2-cyclin E complex |
| cyclin A | the protein that partners with CDK 2 to promote the S phase |
| state of CDK 4/6 in early G1 | p16 (a competitive inhibitor) is bound, so CDK4/6 cannot bind cyclin D. It is inactive. |
| state of Rb in early g1 | Rb is bound to TFE2F |
| state of TFE2F in early g1 | Rb inhibits TFE2F, so it is inactive. |
| what event starts the g1 checkpoint? | cell growth factors trigger cyclin D synthesis |
| how is CDK4/6 activated? | cyclin D out-competes p16 and binds to CDK4/6 |
| what is the job of the activated CDK 4/6 w/Cyclin D complex? | phosphorylates Rb |
| what happens when Rb gets phosphorylated by CDK 4/6 | it releases TFE2F |
| what does TFE2F do once it is released? | it becomes active and makes cyclin E (turns on the gene) |
| what complex forms after cyclin E is made? | CDK 2 w/cyclin E |
| what are the two main targets of the CDK 2 w/cyclin E complex? | it phosphorylates Rb (this is the activation step that locks it off) and it phosphorylates p27 |
| what happens when p27 is phosphorylated? | it tags p27 w/ubiquitin (for destruction) |
| what is the result of destroying p27? | it promotes cyclin A production |
| what complex takes over to promote S phase? | CDK 2 w/ cyclin A |
| how does CDK 2 w/cyclin A promote S phase? | by regulation of transcription factors and regulation of per-replication complexes. |
| p16 binds CDK4/6= | inactive CDK4/6 |
| Rb binds TFE2F | inactive TFE2F |
| what initiates the 'go' signal? | growth factors trigger cyclin D production |
| what is the first step of CDK4/6 activation? | cyclin D out-competes p16 |
| what happens after cyclin D out-competes p16? | answer CDK4/6 activates |
| what does actice CDK 4/6 do to the Rb-TFE2F complex? | Phosphorylates Rb, which releases TFE2F --> active |
| what is the immediate action of active TFE2F? | active TFE2F transcribes Cyclin E |
| what complex forms after cyclin E is transcribed? | cyclin E binds CDK2-->active. |
| what are the two targets of the cyclin E binds CDK2=active complex? | phosphorylate Rb and phosphorylate p27 |
| what is the result of phosphorylating p27? | it uniquitinates cyclin D |
| what is the result of ubiquitination in the transition to S? | promotes cyclin A production |
| what is the final push into S phase? | active cyclin A CDK2 promotes TFs (transcription factors) for DNA replication and S phase. |
Created by:
user-1999447