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162e3: Genetics

QuestionAnswer
Gregor Mendel (1865) Genetics traits are passed on.
"Gene" concept Gregor Mendel
Correns, De Vries and Tschermak Plant geneticist work duplicated an unknown Austrian monk's work (Mendel)
Griffith (1928) Pathogenic/non-pathogenic
Transformation Griffith (1928)
Avery, MacLeod and McCarty Purified the transforming factor . . . DNA
Hershey and Chase (1952) Bacteriophage, DNA
Berg and Mertz (1972) First recombinant DNA (Endonuclease, EcoR1)
SV40+P22 DNA Ligase Berg and Mertz
Boyer, Cohen and Chang (1973) Isolate Kanamycin' resistance gene (EcoR1)
Added to Tetracycline' plasmid Boyer, Cohen and Chang
Transformed E. Coli (dual antibiotic) Boyer, Cohen and Chang
Genetech, Inc. (1977) Human gene expressed in bacteria (Somatostatin - growth hormone inhibiting hormone)
Palmiter and Brister (1982) Microinjected rat growth hormone gene into mice w/dwarfism. First "cure"
Anderson, Blaese and Culver (1990) First human gene therapy.
Corrected Adenosine deaminase gene in WBC Anderson, Blaese and Culver
Genome Sequences Everything
Ian Wilmut (1997) Cloned Sheep (Dolly)
Polly and Molly Ian Wilmut (1997)
Ingo Potrykus and Peter Beyer (2000) Golden Rice
Beta carotene rice Ingo Portykus and Peter Beyer
Did Mendel call his discovery "genes?" What was his profession? No, he never used that term; he called them factors. He was a monk.
What percentage of DNA is genes? 1.5%. 98.5% of DNA is not genes.
How did Mullis come up with PCR? He wanted to go surging, thought about PCR, tried it, and won the Nobel Prize.
4 Facts about PRIMERS: 1) Add heat to separate DNA. 2) Bond to each side for specific replication. 3)16-22 base pairs long. 4)Degenerate PRIMERS are non-specific and generalized.
Created by: user-1999447
 

 



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