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BIOL 1101 Exam Three

In-Class Notes, Translation

QuestionAnswer
What is the function of the small subunit holds the mRNA in place during translion
What is the function of the large subunit peptide bonds form
What is the function of the ribosomal RNA peptide bond formation, ribosome is a ribozyme
When does transcription and translation occur in bacteria happens at the same time, ribosome translates mRNA as its being synthesized by RNA polymerase
What is the entire complex of transcription and translation in the bacteria called polyribosome
When and where does transcription and translation occur in eukaryotes mRNA synthesized and processed in nucleus, translation by ribosomes are done in the cytoplasm
What is tRNA secondary and tertiary struture of RNA
What is aminoacyl-tRNA synthase enzyme that "changes" tRNA with additional amino acids, uses ATP->AMP
What happens at the start of translation for prokaryotes Shin-Delgarno Sequence - ribosome binding site before start codon next to small ribosomal subunit
What happens at the start of translation for eukaryotes 5' cap of mRNA is binded to by small subunit with charged initiator tRNA and initiation factors
How does the small ribosomal subunit function at the start of translation for eukaryotes tries to find AUG start codon -> initiation factor leave -> large subunit of ribosome comes in
What happens at elongation tRNA binds with mRNA -> peptide bond forms -> large subunit moves -> small subunit moves
What happens when peptide bonds forms at elongation? breaking amino acid/tRNA bond AND amino acid joins to peptide chain at the SAME TIME
When does termination phase start? when A site encounters a stop codon
What is the release factor at termination? next to stop codon, looks like tRNA but is a protein
What is the function of a release factor at termination? break peptide bond through hydrolysis linking tRNA in P site with polypeptide chain
Created by: goldengalleon
 

 



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