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BIOL 1101 Exam Three

In-Class Notes, Transcription

QuestionAnswer
Which strand of DNA are templates for transcription in bacteria? both strands can be template strands
What does minus mean? upstream of the transcription
What does plus mean? downstream of the promoter
Where does the promoter start in bacteria -10 to -35
What binds to the promoter to start transcription in bacteria? sigma factor
What is the holoenzyme sigma factor AND all other subunits
What is Rho protein recognizes and binds to a sequence on RNA (free energy from ATP -> ADP)
How does Rho-independent termination work? big protein on the end breaks as only A-T bonds, less h-bonds present
How does Rho-dependent termination work? DNA becomes double stranded at the end, destabilizes the 3' end, detaches
What is rifampin an antibiotic that can bind to the B-subunit and stop elongation
What is L511P mutation? leucine and proline amino acid mutation that disrupts rifampicin for resistance
What is Q513L mutation? glutamine and leucine amino acid mutation that disrupts rifampicin for resistance
What is the difference between prokaryotes and eukaryotes in mRNA? Prokaryotes - 3-P, ribosome binding sites Eukaryotes - g+CH3 and 3-P, exons and introns
What are the main components of eukaryotic transcriptional processing? introns are sliced out, 5' capping, 3' polydentation, protein is translated from mRNA
What is the TATA box promoter where transcription starts
What makes the transcription factor complex? transcription factors, starts RNA transcription
What is the C terminal domain (CTD)? part of RNA Polymerase III, interacts with newly made RNA, capping splicing, poly-A tail, and termination
How does capping work? phosphatase removes phosphate, guanyl transferase puts a g on backwards, methyltransferase adds methyl group
Which RNA products are capped and polydentated? only RNA Polymerase II
What carries out splicing? snRNA/protein complexes
What is the process of splicing? HO binds to one end of the intron, -OH of exon binds to other end of exon (after intron), 2nd phosphoryl-transfer by lariat enzyme
What happens at termination? sequence signals termination, adds -OH, poly-A addition polymerase does NOT need a template
Created by: goldengalleon
 

 



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