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Lab Quiz some number
| Question | Answer |
|---|---|
| Why is optocin susceptibility used as a laboratory test | to differentiate strep pneumoniae from other streptococci. |
| What is the chemical equation of the urease test? | urea + H2O + Urease --> 2NH3 + CO2 |
| How does the CAMP test work? | Some Strep strains (group B) make "CAMP factor" that help other bacteria lyse RBCs better. If there is a large arrowhead shape of RBC lysis in yellow where the bacterial unknown crosses with the S. aureus, it is positive for the CAMP factor. |
| why use a CAMP test? | to differentiate strep agalactiae from other Streptococci |
| How does MacConkey agar work? | differential and selective medium contains lactose, bile salts, neutral red, and CV. bile salts and CV inhibit growth of G+. neutral red dye=pH indicator. colorless above 6.8 and red below 6.8. ferment lactose colonies turn pink. |
| How does optochin work (mechanism of action) | disrupts ATP synthase activity |
| What is the CAMP factor? | a hemolytic factor that acts synergistically with the beta-hemolysin of S. aureus. |
| what color is a positive urease test? | pink |