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ppt 13metabolism

ppt 13 Glucose Metabolism Glycolysis

QuestionAnswer
The last 5 steps of glycolysis are energy payoff phase
What is step 6? GAP Dehydrogenase Reaction
In step 6 phosphate does not come from ATP ? True
In step 6 what is reduced? NAD+ to NADH+H+
Step 6 is what 2 type of reactions phosphorylation and oxidation-reduction
Step 6 Glyceraldehyde-3-phosphate ---> 1,3-Bisphosphoglycerate
Step 7 is? Phosphoglycerate Kinase Reaction
Step 7 1,3-Bisphosphoglycerate ---> 3-phsphoglycerate
In step 7 what is formed? ATP : substrate level phosphorylation
Step 8 3-phosphoglycerate ---> 2-phosphoglycerate
What happens in step 8 phosphate gets moved to C-2
Step 8 is? phosphoglycerate mutase reaction
ATP is formed when this enzyme _____ converts 1,3-bisphosphoglycerate to 3-phosphoglycerate phosphoglycerate kinase
what is net production of ATP from glycolysis? 2
What is the total of glycolysis ATP? 4
Isomerization of 3-phosphoglycerate occurs via an active site __________ residue? His
Step 9 is? Enolase Reaction
Step 9 2-phosphoglycerate ---> phosphoenolpyruvate
In step 9 what does enolase catalyzes? dehydration reaction
What produced is produced in Step 9? H2O
Step 10 is? Pyruvate Kinase Reaction
Step 10 phosphoenolpyruvate ---> pyruvate
What is formed in step 10? ATP : substrate level phosphorylation
Step 10 is reversible or irreversible? Irreversible : -61.9 KJ . mol
Step 1 Hexokinase Glucose ----> Glucose 6-P inhibited by high conc of G 6-P (product inhibition)
Step 3 Phosphofructokinase Fructose 6-P ---> Fructose 1,6-bis P is the most regulated step
Step 3 Phosphofructokinase activated by? Fructose 2,6-Bis P ; ADP
Step 3 Phosphofructokinase inhibited by? ATP, phosphoenolpyruvate, citrate
Step 10 Pyruvate Kinase phosphoenol pyruvate ---> pyruvate is activiated by ? fructose 1,6-bis p ; feed forward regulation
Glycolysis occurs in 10 steps steps 1-5 = energy investment steps 6-10 = energy payoff
Steps 1-5 ______ ATP are consumed 2
steps 6-4 ______ ATP are produced 4
Net production is ______ units per molecule of glucose 2 ATP
Glucose (6-carbon molecule) is broken down into ? two 3-carbon molecules (pyruvate)
Electron carriers are ___________ reduced
What happens to pyruvate? Acetyl-CoA, Lactate, Ethanol +CO2, Oxaloacetate
During exercise pyruvate can be temporarily converted to lactate? True ; anaerobic glycolysis
Pyruvate can be further oxidized? Yes by acetly group getting picked up by CoA
Phosphoenolpyruvate acts as inhibitor through a negative feedback mechanism for ? phosphofructokinase
Pyruvate is a precursor of __________? oxaloacetate
oxaloacetate is a metabolite used in? -the citric acid cycle -gluconeogenesis
oxaloacetate is also an intermediate in? amino acid synthesis
lactate dehydrogenase catalyzes the following conversion pyruvate to lactate
which of the following enzymes catalyzes irreversible reactions in glycolysis? -phosphofructokinase -hexokinase -pyruvate kinase
regulation of glycolysis? Hexokinase, phoshphofructokinase, pyruvate kinase
How many irreversible reactions are in the energy payoff phase ? One irreversible reaction
How many irreversible reactions are in the energy investment phase? Two irreversible reactions
This enzyme in the second half of glycolysis is responsible for the first substrate level phosphorylation event to generate ATP and 3 phosphoglycerate phosphoglycerate kinase
Created by: sofialorena
 

 



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