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Lab exam 2
| Question | Answer |
|---|---|
| Gram stain purpose | to identify Gram + and Gram - bacteria |
| primary stain (gram) | crystal violet |
| mordant (gram stain) | iodine |
| differential step (gram stain) | decolorizer |
| counterstain (gram) | safranin |
| Capsule stain general procedure | Stain outside w/ negative stain (primary stain), then counterstain that goes into the organism (clear area around organism will be the capsule) |
| primary stain (capsule) | congo red |
| Secondary stain (capsule) | Maneval's solution |
| Which stain in a capsule stain colors the background | congo red |
| Which stain colors the cell in a capsule stain | maneval's solution |
| What does oxidase test identify | identifies bacteria that have cytochrome oxidase |
| Coagulase test test for | tests for the presence of coagulase enzymes |
| How does the coagulase test work? | There is rabbit plasma in a test tube, and then an inoculation with a microbe. If there is clumping, the microbe has the coagulase enzyme. |
| What does bacitracin do | interferes with transport of peptidoglycan subunits across the cytoplasmic membrane |
| what is a capsule | material surrounding the cell outside the cell wall consisting of polysaccharides or protein. It serves to protect the cell. |
| Mannitol salt plate | selective and differential; high salt concentration with phenol red to indicate a pH change |
| Alpha hemolysis | partial hemolysis of RBCs |
| Beta hemolysis | complete lysis of red blood cells |
| gamma hemolysis | no hemolysis |
| catalase reaction equation | hydrogen peroxide → water + oxygen |
| what does a positive oxidase test look like? | dark blue/purple within 20 seconds |
| What does bacitracin identify | used to differentiate and identify beta-hemolytic Group A strep from other beta-hemolytic strep. It also differentiates the genus staphylococcus (R) and micrococcus (S) |
| different types of coagulase | free and bound. Free is an extracellular enzyme. Bound is a part of the bacterial cell wall. |
| What does coagulase identify | differentiates S. arueus from other gram positive cocci |
| What does oxidase test test for | used to identify bacteria containing the respiratory enzyme cytochrome c oxidase. |
| What does the capsule stain test for | differential stain used to detect cells capable of making an extracellular capsule |
Created by:
Kayla_K