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BIOL 1101 Exam Two
In-Class Notes, Cytoskeleton
| Question | Answer |
|---|---|
| How does vesicle formation happen | Coat components aggregate to donor compartment by binding to G protein, another G protein undergoes shape change and separates budding vesicle from donor compartment |
| How does vesicle fusion happen | Rab protein leads vesicle to destination, where V-SNARE and T-SNARE protein leads to fusions of the vesicle to the target compartment, move through fibers of cytoskeleton |
| What are microfilaments and their function | muscle contraction, motility, two strands intertwined |
| What are intermediate filaments and their function | fiber wound into thicker strands, maintains cell shape by resisting tension |
| What are microtubules and function | maintain cell shape by resisting compression, movement of chromosomes during cell division |
| Which filaments are dynamic | microfilaments (actin) and microtubules (tubin), monomers assemble and disassemble easily |
| What is g-actin | globular actin, monomer units |
| What is f-actin | fibrillar actin, polymeric units |
| How does actin assemble | actin bound to ATP, polymerize with the + end of microfilmanet |
| How does actin disassemble | actin bound to ADP, dissociate from other monomers, occurs at - end |
| How does microtubule assemble | beta-tubulin bound to GTP, dimers polymerize |
| How does microtubule disassemble | beta-tubulin bound to GDP, microtubule comes apart |
| What is different about microtubules compared to microfilmants in terms of assembly | microtubules can grow and shrink from both sides |