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Microbiology

Chapter 4

QuestionAnswer
Bacteria and Archaea generally multiply by the process of .... binary fission
Binary Fission a process in which cell increases its size replicates its DNA and then divides
generation time the time it takes for population to double in number
To calculate how many bacterial cells will be present in a product after a certain amount of time two factors must be known 1. the inital number of cells in the orignal population 2. the number of times the cells will divide during the stated period.
N(t)=N(o)X 2 (n) Nt = number of cells at a given time No= the initial number of cells
Microorganisms can live suspended in aqueous enviroment but must attach in surfaces and live in polymer encased communities called biofilms
biofilm formation begins when ... planktonic cells move to a surface and adhere
biofilm release ... polysaccharides, DNA and other hydrophilic polymers to which unrelated cells may attach and grow.
EPS (extracellular polymeric sub) gives bioflim its slimy appearance
pure culture a population descended from a single cell and therefore contains only one species
can be sucessfully grown in culture 1% of all prokaryotes
aseptic technique a set of procedures used to prevent the accidental intro of unwanted microbes
culture medium hat the cells grow in
Agar polysaccharide extracted from seaweed
agar is resistant to degraduation
agar stays liquid until 45C
Streak Plate Method goal is to reduce number of cells being spread with each series of streaks
once a pure culture has been obtained it can be maintained as a ... sock culture
In labs many microorgainsms are grown on agar plates \, tubes and flask this is considered closed systems or batch systems
Created by: alejac0360
 

 



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