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*NBMNT
for metabolic pathways spicifically cuz u dumb
| Question | Answer |
|---|---|
| What enzyme phosphorylates glucose in the liver? | Glucokinase. |
| What enzyme phosphorylates glucose in muscle? | Hexokinase. |
| What is the rate-limiting enzyme of glycolysis? | Phosphofructokinase-1 (PFK-1). |
| What are the two ATP-producing (substrate-level phosphorylation) steps in glycolysis? | 1,3-BPG → 3-PG; PEP → Pyruvate. |
| What are the 4 possible fates of pyruvate? | Acetyl-CoA, Lactate, Oxaloacetate, Alanine. |
| Which enzyme converts pyruvate to Acetyl-CoA? | Pyruvate Dehydrogenase Complex (PDH). |
| What are the 5 cofactors of PDH? | Thiamine (B1), Lipoic acid, CoA, FAD (B2), NAD⁺ (B3). |
| Where does the TCA cycle occur in the cell? | Mitochondrial matrix. |
| Where is the electron transport chain located? | Inner mitochondrial membrane. |
| What is the final electron acceptor in the ETC? | Oxygen (O₂). |
| How many ATP are produced per NADH in the ETC? | ~2.5 ATP. |
| How many ATP are produced per FADH₂ in the ETC? | ~1.5 ATP. |
| Which shuttle system moves NADH into mitochondria in the heart and liver? | Malate-aspartate shuttle. |
| Which shuttle system moves NADH into mitochondria in muscle? | Glycerol-3-phosphate shuttle. |
| What enzyme breaks α-1,4 bonds in glycogen during glycogenolysis? | Glycogen phosphorylase. |
| Which enzyme removes glycogen branches (α-1,6 bonds)? | Debranching enzyme. |
| Which two amino acids are purely ketogenic? | Leucine and Lysine. |
| Which amino acid is both glucogenic and ketogenic? | Isoleucine (also phenylalanine, tyrosine, tryptophan). |
| What transports long-chain fatty acids into mitochondria for β-oxidation? | Carnitine shuttle. |
| What triggers ketone body formation during fasting? | Low oxaloacetate (used in gluconeogenesis), causing Acetyl-CoA to form ketones. |