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anaerobes ****
| Question | Answer |
|---|---|
| obligate anaerobes can not tolerate _____ | oxygen: it has a toxic effect |
| obligate anaerobes lack ____ to break down toxic products | enzymes |
| Organisms that exist outside of the bodies of animals are called______ | Exogenous |
| Organisms that exist inside the bodies of animals and become sources of infection are called _______ | Endogenous |
| Anaerobes have _____ odor; Do not grow on ___ culture plates | foul; aerobic |
| Bacteroids spp have a good growth on _______ agar | bile-esculin agar (BBE) |
| Anaerobes have a Double zone of hemolysis on _____agar; Brick-red fluorescence on ______ agar | BAP; KVLB |
| Acceptable Samples for anaerobs are _____ | Body fluids; Abscesses; Deep aspirates of wounds and soft tissue infections; Transtracheal aspirates Direct lung puncture; Suprapubic bladder aspirates; Normally sterile sites; Endometrial sites |
| Unacceptable Samples for anaerobs are _____ | Saliva expectorated sputum; Exudates and other materials collected by swabs from superficial wounds; Nasotracheal aspirates; Vaginal/cervical/urethral secretions; Voided or catheterized urine; |
| Enriched primary isolation media for ana are ______ | Shaedler blood agar; Brucella blood agar |
| Selective isolation media for ana are ___ | KV blood agar (kanamycin-vancomycin) PEA, PV( paramomycin-vancomycin) Bile-containing agar |
| Enriched Broths for ana are ___ | Enriched thioglycolate broth Chopped meat glucose broth |
| Jar techniques for ana are ___ | Evacuation-replacement; Gas Pak |
| Methods of performing anaerobic cultures are _____ | Anaerobic chamber or glove box; Jar techniques; Biobags |
| Anaerobic jars: Inoculated plates, gas-generating envelopes, and an ______ are placed within the jar | indicator strip |
| Anaerobic pouch: After inoculated plates are added to the pouch, an _____ system is initiated | oxygen-removal |
| GP bacilli with spore is ______spp | Clostridium |
| When Porphyromonas and Prevotella species are exposed to UV light, they produce | Brick-red fluorescence |
| Frequently Encountered Anaerobes are _____ | Clostridium sp.; Bacteroides spp.; Fusobacterium spp.; Prevotella and Porphyromonas; Veillonella spp.; Peptococcaceae |
| Most clinical specimens isolated are _______ | Bacteroids spp 43% |
| Clostridium tetani show ____ spores ; Clostridium sordelii with Clostridium sordelii with ___ spores spores | terminal; Clostridium sordelii with subterminal spores |
| Clostridium perfringens showing a positive ________ reaction on egg yolk agar | lecithinase |
| Double-zone of hemolysis is characteristic of ________ | C. perfringens |
| Gram-Positive non–spore-Forming anaerobic bacilli are _______ | Actinomyces israelii; Mobiluncus curtsii; Propionibacterium acnes |
| Most frequently GN bacilli isolated from cultures is ____ | Bacteroides fragillis 61% |
| Bacteroides fragilis grow on a KVLB agar (white) and BBE agar(______) | black |
| Gram-stained morphology of _______show the fusiform shape of the organism | Fusobacterium nucleatum |
| Gram-stained morphology of Fusobacterium mortiferum show its _____ | pleomorphism |
| Anaerobic Gram Positive Cocci are _______ | Peptostreptococcus sp; Peptococcus sp |
| Gram-stained appearance of Peptostreptococcus sp. show the typical _____ formation | chain |
| It is a weakly catalase positive, rarely isolated from clinical specimens | Peptococcus niger |
| To find if anaerobic bacteria is present always do _____plates | oxigen tolerance plates(one at 35 grade C, and one with CO2) |
| What measures the concentration of gases? | gas-liquid chromatography |
| Campilobacter is microaerophilic, need a low _ | CO2 |
| Propionicbacterium in skin causes ____ | acnee |
Created by:
tatianat