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Staining

QuestionAnswer
Define and give examples of absorption Tissue becomes penetrated by, or absorbs a dye solution and becomes colored without any change or chemical rxn occuring.
Adsorption accumulation of a stain by the surface of a tissue component. Influenced by oppositely charged ions
Mordant reagent used to link the stain, or dye molecules, to the tissue. Many mordants are metal
Basophilic Acidic substance (anionic, negatively charged) that is easily stainable with basic dyes
Acidophilic Basic substance (Cationic, positively charged) that is easily stainable with acidic dyes
Auxochrome chemical group present in a dye that causes it to bind to certain tissue elements. This group can develop a charge and thus bind to oppositely charged groups present in the tissue
Chromophore the chemical grouping that bestows the property of color on a compound
Polychrome Describes a single dye solution that stains different components different colors by phenomena other than metachromasia
Lake combination of a mordant with a dye. The dye-lake can bind to tissue components and form an insoluble colored deposit.
Dye Physiological Properties -Fluorescent -Metachromatic -Leuko
Natural Dyes CIBOSH Carmine Indigo Brazilian Orcein Saffron Hematoxylin
Indigo From the indigo plant Blue color Can be synthesized artificially
Carmine Very little affinity for tissue usally add metal salt (iron, aluminum) Carmine + aluminum= Mucicarme (stains mucin in goblet cells)
Orcein Boil to create Orcinol Orcinal + air + ammonia=Orcein Brown connective tissue stain
Brazilian Used as a red nuclear stain Brazilwood tree
Hematoxylin Hematoxylin + air=hematin Hematein + metal salt= Biological stain
Chromogen Quinone + Chromophores
Dye Quinone + Chromophore + Auxochrome
Leuko Dyes Dyes that undergo oxidation/reduction to become colorless and then reduction/oxidation to have color return Ex: Schiffs
Substantive-Direct Dye binds directly to tissue
Adjective-Indirect Tissue treated first, then dye is attached
Impregnation Metallic dye deposited onto the structure it is staining, not in
Created by: rebtsango
 

 



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