click below
click below
Normal Size Small Size show me how
MICROBIOLOGY
sample questions test 4
| Question | Answer |
|---|---|
| In practical terms, which of the following statements provides the definition of sterilization? | sterilization eliminates all organisms and their spores or endospores. |
| Which of the following substances or processes kills microorganisms on laboratory surfaces? | disinfectants |
| Which of the following terms best describes the disinfecting of cafeteria plates? | sanitization |
| The microbial death rate is used to measure ______. | All of these |
| Which of the following statements is true concerning the selection of an antimicrobial agent? | All of these |
| The spores of which organism are used as a biological indicator of sterilization? | Bacillus stearothermophilus |
| A company that manufactures an antimicrobial cleaner for kitchen counters claims that its product is effective when used in a 50% water solution. By what means might scientists best verify this statement? | In-Use test |
| Which of the following items functions most like an autoclave? | A pressure cooker |
| The preservation of beef jerky from microbial growth relies on which method of microbial control? | Desiccation |
| Which of the following types of radiation is more widely used as an antimicrobial technique? | Electron beams |
| Which of the following substances would most effectively inhibit anaerobes? | Hydrogen peroxide |
| Which of the following adjectives best describes a surgical procedure that is free of microbial contaminants? | Aseptic |
| Biosafety Level 3 includes ___. | All of these |
| A sample of E. coli has been subjected to heat for a specified time, and 90% of the cells have been destroyed. Which of the following terms best describes this event? | Decimal reduction time |
| Which of the following substances is least toxic to humans? | Hydrogen peroxide |
| Which of the following chemicals is active against bacterial endospores? | Ethylene oxide |
| Which of the following disinfectants acts against cell membranes? | Phenol |
| Which of the following disinfectants contains alcohol? | A tincture of bromide |
| Which antimicrobial chemical has been used to sterilize spacecraft? | Ethylene oxide |
| Which class of surfactants is most soluble in water? | Soaps |
| Diffusion and dilution tests that expose pathogens to antimicrobials are designed to ___. | Both 2 and 3 are correct. |
| In a Kirby-Bauer susceptibility test, the presence of a zone of inhibition around disks containing antimicrobial agents indicates ___. | That the microbe does not grow in the presence of the agents |
| The key to successful chemotherapy is ___. | Selective toxicity |
| Which of the following statements is relevant in explaining why sulfonamides are effective? | Humans and microbes use PABA differently in their metabolism. |
| Cross resistance is ___. | Resistance to one antimicrobial agent because of its similarity to another antimicrobial agent |
| Multiple-drug-resistant microbes ___. | frequently develop in hospitals |
| Which of the following is most closely associated with a beta-lactam ring? | Penicillin |
| Drugs that act against protein synthesis include ___. | Aminoglycosides |
| Which of the following statements is false concerning antiviral drugs? | Macroglide drugs block attachment sites on the host cell wall and prevent viruses from entering |
| PABA is ___. | Used to synthesize folic acid |
| Which of the following is the most likely number of base pairs in a bacterial chromosome? | 4,000,000 |
| Which of the following is a true statement concerning the bacterial chromosome? | They are located in the cytosol. |
| A plasmid is ___. | extrachromosomal DNA |
| Which of the following forms ionic bonds with eukaryotic DNA and stabilizes it? | histone |
| Nucleotides used in the replication of DNA ___. | All of these |
| Which of the following molecules functions as a “proofreader” for a newly replicated strand of DNA? | DNA polymerase III |
| The addition of -CH3 to a cytosine molecule after DNA replication is called ___. | Methylation |
| In translation, the binding site through which the tRNA molecules leave is called the ___. | E site |
| The Ames test ___. | uses auxotrophs and liver extract to reveal potential mutagens |
| Which of the following methods of DNA repair involves enzymes that recognize and correct nucleotide errors in unmethylated strands of DNA? | mismatch repair |
| Which of the following is not a mechanism of natural genetic transfer and recombination? | transcription |
| Cells that have the ability to take up DNA from their environment are said to be ___. | competent |
| Which of the following statements is true? | Conjugation requires a sex pilus extending from the surface of a cell. |
| Which of the following are called “jumping genes”? | transposons |
| Although cells P and Q are totally unrelated, cell Q receives DNA from cell P and incorporates this new DNA into its chromosome. This process is ___. | horizontal gene transfer |
| Which of the following is part of each molecule of mRNA? | codon |
| A nucleotide is composed of ___. | All of these |
| In DNA, adenine forms ___ hydrogen bonds with ___. | two; thymine |
| A sequence of nucleotides formed during replication of the lagging DNA strand is ___. | an Okazaki fragment |
| Which of the following is not part of an operon? | origin |
| Repressible operons are important in regulating prokaryotic ___. | RNA transcription |
| Transcription produces ___. | RNA molecules |
| Ligase plays a major role in ___. | lagging strand replication |
| Before mutations can affect a population permanently, they must be ___. | inheritable |
| The trp operon is repressible. This means it is usually ___ and directly controlled by ___. | active; repressor |
| Which of the following statements is true concerning recombinant DNA technology? | It involves modification of an organism's genome. |
| A DNA gene synthesized from an RNA template is ___. | complementary DNA |
| After scientists exposed cultures of Penicillium to agents X, Y, and Z, they examined the type and amount of penicillin produced by the altered fungi to find the one that is most effective. Agent X, Y, and Z were probably ___. | mutagens |
| Which of the following is false concerning vectors in recombinant DNA technology? | Vectors must contain genes for self-replication. |
| Which recombinant DNA technique is used to replicate copies of a DNA molecule? | PCR |
| Which of the following would be most useful in following gene expression in a yeast cell? | DNA microarray |
| Which of the following techniques is used regularly in the study of genomics? | Fluorescent nucleotide bases are sequenced. |
| Restriction enzyme HhaI ___. | All of these |
| Which application of DNA technology involves the production of a distinct pattern of DNA fragments on a gel? | Genetic fingerprinting |
| A DNA microarray consists of ___. | single-stranded DNA localized on a substrate |