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Venipuncture
| Question | Answer |
|---|---|
| What is the "order of draw"? | It refers to the specific sequence in which blood collection tubes are filled during venipuncture. |
| Why is following the order of draw important? | To prevent cross-contamination of additives that can alter lab test results. |
| What is the purpose of blood culture tubes (yellow or yellow-black top)? | To detect bacterial or fungal infections in the blood. |
| Why are blood culture tubes drawn first? | To avoid contamination from additives that could inhibit microbial growth. |
| What is the purpose of coagulation tubes (light blue top)? | Used for coagulation studies like PT and aPTT. |
| What additive is in the light blue top tube? | Sodium citrate. |
| Why is the light blue top tube drawn second? | To avoid interference with coagulation tests from other additives. |
| What is the purpose of serum tubes (red, gold, or tiger top)? | Used for chemistry, serology, and immunology tests. |
| What additives are in gold/tiger top tubes? | Clot activator and serum separator gel. |
| Why are serum tubes drawn third? | They lack anticoagulants that could interfere with prior tubes. |
| What is the purpose of heparin tubes (green top)? | Used for plasma chemistry tests and blood gas analysis. |
| What additive is found in green top tubes? | Lithium or sodium heparin. |
| Why are heparin tubes drawn fourth? | Heparin can affect coagulation and serum tests if drawn earlier. |
| What is the purpose of EDTA tubes (lavender or purple top)? | Used for hematology tests such as CBC. |
| What additive is in EDTA tubes? | Ethylenediaminetetraacetic acid (EDTA). |
| Why are EDTA tubes drawn fifth? | EDTA has strong anticoagulant effects that can interfere with other tests. |
| What is the purpose of oxalate/fluoride tubes (gray top)? | Used for glucose and lactate testing. |
| What are the additives in gray top tubes? | Potassium oxalate and sodium fluoride. |
| Why are gray top tubes drawn sixth? | To preserve glucose levels without interference from prior tube additives. |
| What are some examples of "other tubes" drawn last? | Royal blue, tan, yellow-top ACD tubes. |
| Why are specialized tubes drawn last? | To prevent contamination that could interfere with their specific test purposes. |