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DNA replication HH
Learn about DNA replication
| Question | Answer |
|---|---|
| Name the bond between the bases. | Hydrogen |
| Between which two parts of the nucleotide is the back bone. | Sugar and phosphate |
| The 5' end of the nucleotide is the | phosphate |
| The 3' end of the nucleotide is the | deoxyribose |
| Name the 3 parts to a DNA nucleotide | 1. phosphate 2. base 3, deoxyribose |
| How many strands are found in DNA. | 2 strands |
| Name the 3D shape of DNA | double helix |
| Name the complementary DNA base pairs | adenine binds with thymine guanine binds with cytosine |
| Name the type of chromosomes found in prokaryotes | circular |
| Name the type of DNA found in prokaryotes in addition to a single circular chromosome | plasmids |
| Name the type of chromosome found in the nucleus of eukaryotes | linear chromosome |
| Name the type of chromosome found in chloroplasts/mitochondria of eukaryotes. | circular chromosomes |
| Name the type of molecule that linear chromosomes are tightly coiled around in the nucleus. | histone proteins |
| Name the eukaryote that has plasmids | |
| Name the first stage of DNA replication. | Double helix unwinds |
| State what happens in DNA replication after the double helix unwinds. | Hydrogen bond break between the bases. |
| State the role of primers in DNA replication. | Bind/anneal to DNA template OR start DNA replication by DNA polymerase |
| State the role of DNA polymerase in DNA replication. | Adds free complementary DNA nucleotides to 3'end of new strand |
| In what direction does DNA polymerase replicate on the new strand. | From 5' to 3' end OR onto the 3' end |
| Describe what is meant by DNA being anti parallel. | 1 strand runs from 5' to 3' 1 strand runs from 3' to 5; |
| Compare leading and lagging strand in terms of their replication. | leading strand replicates continuously whilst the lagging strand is replicated in fragments. |
| Explain why there is a lagging strand in DNA replication. | As DNA polymerase only replicates from 5' to 3' end on the new strand OR only adds onto the 3' end of the new strand. |
| State what process follows DNA replication. | Cell division/mitosis |
| Explain why DNA replication must come before mitosis. | To ensure each daughter cell receives the same number of chromosomes as the original cell. |
| Put the following stages in order. 1. primer binds to DNA template 2. DNA polymerase adds free DNA nucleotides to 3' end 3. Hydrogen bonds break between the bases | 1. Hydrogen bonds break between the bases 2. primer binds to DNA template 3. DNA polymerase adds free DNA nucleotides to 3' end |
| State the name of the molecule that joins the lagging fragments together | Ligase |
| State two molecules needed for DNA replication. | 1. DNA template 2, free DNA nucleotides 3. ligase 4. DNA polymerase 5. ATP 6. Primers |