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PCR

learn more about PCR

QuestionAnswer
State the function of PCR To amplify DNA
Describe one practical application of PCR 1. solve crimes 2. paternity tests 3. diagnose genetic disease
Describe what happens at 92-98 degrees strands seperate OR hydrogen bonds break between bases
Describe what happens at 50-65 degrees primer binds/anneals to target DNA OR primers start DNA replication by DNA polymerase
Describe what happens at 70 -80 degrees DNA polymerase replicates the DNA
At what temperature does the primer bind to the target DNA Any value between 50-65
At what temperature does the heat tolerant DNA polymerase replicate the DNA. Any value between 70-80
At what temperature do the two strands of DNA separate/denature. Any value between 92-98
In terms of heating and cooling state whether step 1, 2 and 3 are heating or cooling. step 1 - heating step 2 - cooling step 3 - heating
Explain why a heat tolerant Taq polymerase is needed. To prevent the enzyme denaturing at the high temperature of PCR
Explain why there is no lagging strand in PCR DNA is separated into separate strands
How many primers are needed in PCR. Two in total (one for each strand )
Is ligase needed for PCR. NO ( there is no lagging strand as DNA is separated into single strands)
If there are 100 molecules of DNA at the start how many DNA molecules are present after 3 cycles. 800
If there are 400 molecules of DNA at the start how many DNA molecules are present after 15 minutes if a cycle lasts 3 minutes. 12800
State two substances needed for PCR 1. free DNA nucleotides 2, primers 3, DNA polymerase 4, DNA sample 5. ATP
Created by: brightminds
 

 



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