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Micro/Linne Quest
MICROBIOLOGY Linne` Questions
| Question | Answer |
|---|---|
| 1. Microbiology laboratory-acquired infections from an aerosol: | Are associated with improper venting of air in the laboratory setting. |
| 2. Prevention of aerosolization can best be accomplished by:` | Using a biological safety cabinet when working with specimens or when forming aerosols. |
| 3. Media that contain dyes, antibiotics, or other chemical compounds that inhibit certain bacteria while allowing others to grow are called: | Selective media |
| 4. Media that contain factors (e.g. carbohydrates) that give colonies of particular organisms distinctive characteristics are called. | Differential media |
| 5. Media that are used to permit the normal rate of growth of most nonfastidious organisms are called: | Supportive media |
| 6. Which of the following media is not a selective medium? | sheep blood agar |
| 7. Which of the following media is used to promote the growth of gram-negative organisms while inhibiting the growth of gram-positive organisms? | MacConkey agar |
| 8. which of the following media is used to promote the growth of Neisseria gonorrhoeae and Neisseria meningitidis? | Thaye-Martin agar |
| 9. automated microbiology systems have generally been designed to replace: | Manual procedures that are repetitive and that are performed daily on a large number of specimens. |
| 10. The lowest concentration of antimicrobial agent that will visible inhibit the growth of the organism being tested is known as the: | Minimum inhibitory concentration (MIC |
| 11. Use of triple sugar iron agar or Kligler's iron agar can identify all but which one of the following characteristica of members of the Enterobacteriaceae family (common enteric intestinal pathogens)? | ability to produce ammonia |
| 12. Pathogenic shigella spp. characteristically are: | Urease negative |
| 13. which of the following quantitative urine culture results would be considered clinically significant for a urinary tract infection in an asymptomatic patient? | 10 power of 5 CFU/mL urine |
| 14. Which of the following organisms can be recognized by its spreading growth apperance on sheep blood agar? | Proteus spp. |
| 15. Urogenital swabs to be cultured for gonococci should be plated onto culture media: | Immediately: at the bedside preferable |
| 16.In culturing a throat swab for group A B-hemolytic streptococci testing, which of the following media is preferred? | Sheep blood agar |
| 17. What is the purpose of making cuts in the sheep blood agar when a throat culture is plated? | To observe the appearance of any hemolysis present. |
| 18. In some people, untreated pharyngitis infections with group A B-hemolytic streptococci can eventually result in : | Acute glomerulonephritis |
| 19.In observing a sheep blood plate inoculated with a throat swab and showing the presence of alph or green hemolysis after incubation, what test determines if organism is viridans streptococci, part of normal throat flora, or Streptococcus Pneumoniae? | Bile solubility test, in which most s. pneumoniae colonies would be dissolved by the reagent used and inhibited by optochin disk. |
| 20. In identifying the presence of most group A B-hemolytic streptococci, versus those that are o-group A, which of the following test can be done? | PYR test, in which most group A B-hemolytic streptococci colonies would produce a bright red color after addition produce a bright red color after addition of the PRY reagent tothe filter paper. |
| 21. Which of the following intimicrobial agents is used to inhibit mompathogenic fungi from growing in media that have been designed to promote growth of pathogenic fungi (e.g. Mycosel)? | Cyclohximide |
| 22. A Gram-stained sputum smear shows 40 to 50 squamous epithelial cells per low-power (10x) field, along with gram-positive cocci, many gram negative rods, and many gram positive cocci in pairs using the oil immersion objective. Results are reported as? | No report; request another specimen because this one is contaminated with mouth flora (probably saliva, not sputum), as evidenced by the large number of squamous epithelial cells. |
| 23. "Clue cells" ar best seen in which of the following specimens? | Wet preparation of vaginal discharge |
| 24. When antibiotic therapy is needed, specimens for culture and organism identification should be collected: | Before the antibiotic have been administered |
| 25. If the antibiotic does not inhibit the growth of an organism, the organism is said to be which of the following: | Resistant |
| 26. The best method for finding pinworm organisms in children is: | Cellophane tape collection |
| 27. What are the requirements for collection of an appropriate specimen for the detection of chlamydia? | First use a large swab to remove secretions present, then use a second swab to collect the specimen. |
| 28. What are the requirements for collection of an appropriate specimen for the optimal detection of Trichomonas vaginalis? | Examine the collected specimen while it is still fresh, when the organisms are still motile. |
| 30. Collection of fecal samples for identification of intestinal parasites should be done: | Before radiological studies using barium sulfate have been done. |
Created by:
nugentbarbara