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HISTOPATHOLOGY
| Question | Answer |
|---|---|
| METHODS OF FRESH TISSUE EXAMINATION | TEASING / DISSOCIATION SQUASH PREPARATION (CRUSHING) SMEAR PREPARATION FROZEN SECTION |
| A tissue sample is immersed in a watch glass containing isotonic salt solutions, carefully dissected or separated and viewed under the microscope. | TEASING / DISSOCIATION |
| It can be either unstained in Phase Contrast and Brightfield Microscopy or stain with differential dyes | TEASING/DISSOCIATION |
| Small pieces of tissue not more than one mm in diameter is placed in a microscopic slides and forcibly compressed with another slide/cover glass. | SQUASH PREPARATION (CRUSHING) |
| If necessary, a vital stain may be placed at the conjunction of the slide and cover glass, allowing the stain to be absorbed by the tissue in capillary action | SQUASH PREPARATION (CRUSHING) |
| Process of examining the sediment or secretions where a cellular materials are gently spread over a slide by an applicator or making an apposition smear with another slide. | SMEAR PREPARATION |
| A method of fresh tissue examination that is useful in cytological examinations, particularly for cancer diagnosis | SMEAR PREPARATION |
| Using an applicator, it is applied in a direct or zigzag line throughout the slide, attempting to uniformly distribution of the secretions. | STREAKING |
| Too thick and too thin smears should be avoided as it is unsuitable for examination | STREAKING |
| A selected portion of material is transferred to a clean slide and gently spread into a moderate thick film by teasing the mucous strands by an applicator | SPREADING |
| It is tedious but has an advantage in maintaining cellular interrelationship of the material to be viewed. | SPREADING |
| It is recommended for smear preparations of fresh sputum, bronchial aspirates and for thick mucoid secretions | SPREADING |
| A drop of sediments is placed in a slide and facing it with another slide. A slight movement of the two slides are necessary to initiate flow of the specimens. Then two slides are pull apart in an uninterrupted motion | PULL APART |
| This is useful for making smears for thick secretions such as serous fluids, concentrated sputum, enzymatic lavages samples such as gastrointestinal tract and blood smears | SMEAR PREPARATION |
| A special method of smear preparation | TOUCH PREPARATION (IMPRESSION SMEAR) |
| A surface of tissue is pressed over a clean glass slide, allowing the cells to be directly transferred to the slide for examination for Phase contrast or stained for lught microscopic study | TOUCH PREPARATION (IMPRESSION SMEAR) |
| It has an advantage that the cells may be examined without destroying their intercellular relationship, and without separating them from their normal surroundings | TOUCH PREPARATION (IMPRESSION SMEAR) |
| This is utilized when a rapid diagnosis of tissue in question is required and is especially recommended for lipids and nervous tissue elements are to be demonstrated | FROZEN SECTION |
| Most commonly available freezing agents | LIQUID NITROGEN |
| A freezing agents the causes soft tissue liable to crack due to rapid expansion of ice within the tissue, produces ice crystals and freeze artifacts. Also, overcools urgent block causing damage to blocks and blade when processed to minus 70 and below | LIQUID NITROGEN |
| The majority of non-fatty unfixed tissue are sectioned in a temperature between ___________ | -10 to -25 degree celsius |
| Use to freeze small pieces of tissues except for muscle | AEROSOL SPRAYS |
Created by:
kaileayn