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CLSI Order of Draw

QuestionAnswer
Sterile Bottles #1 Color: Yellow Laboratory: Microbiology Additive: SPS (sodium polyanethol sulfonate) Yields: na Inversion Amount: 8-10 Common Tests: blood cultures Additional Information: anaerobic, aerobic; FUO (fever of unknown origin)
Discard Tube #2 Clear or Red Laboratory: Additive: Inversion Amount: Yields: Common Tests: Additional Information: a sample is drawn into this tube and discarded to avoid thrombin and other cells pollution into other samples/tubes
Light Blue Vacutainer #3 Light blue Laboratory: coagulation Additive: sodium citrate (anticoagulant) Inversion Amount: 3-4x Yields: plasma Tests: PT (prothrombin time), PTT (partial), aPTT (activated), PT-INR, DDimer Addl Info: 9:1 (blood:additive), must be full
Red Vacutainer #4 Red Laboratory: Chemistry Additive: none Inversion Amount: none Yields: serum Common Tests: ETOH, pregnancy Additional Information: “plain vacuum tube”, blood clots within 30-60’
Tiger-Top Vacutainer #5 Tiger-Top/SST (serum separator tube) Lab: Chemistry Add: silica (clot activator), thixotropic gel (serum separator) Invers: 8x Yields: serum Tests: BMP (basic metabolic panel), CMP (complete metabolic panel), serum pregnancy, lipids, cholesterol
Mint Green Vacutainer # 6 Mint Green/PST (plasma separator tube) Lab: Chemistry Additive: thixotropic gel (plasma separator), lithium heparin (anticoagulant) Inversion: 8x Yields: plasma Common Tests: LYTES (electrolytes), HCG (qualitative, quantitative), bilirubin
Dark Green Vacutainer #7 Dark Green Laboratory: Chemistry Additive: Sodium (Na) Heparin (anticoagulant) Inversion Amount: 8x Yields: plasma Common Tests: ammonia Additional Information: put on ice
Pink Vacutainer #8 Pink Laboratory: Blood bank Additive: potassium EDTA Inversion Amount: 8x Yields: plasma Common Tests: TSR (type & screen ratio) (A,B,AB,O+/-), cross-match Additional Information: ⅔ or more full
Lavender/Purple Vacutainer #9 Lavender/Purple Laboratory: Hematology Additive: EDTA Inversion: 8x Yields: Plasma Common Tests: ESR (erythrocyte sedimentary rate), sickle cell, CBC (complete blood count), hematocrit, hemoglobin, platelets NEVER centrifuge, ⅔ or more full
Gray Vacutainer #10 Gray Lab: Chemistry Add: 1. Sodium fluoride (antiglycolytic agent - glucose preserver) 2. Potassium oxalate (anticoagulant) Inversion: 8x Yields: Plasma Common Tests: OGTT, LA (lactic acid-oxyge), ETOH Add Info: no alcohol to clean (false +)
Royal Blue Vacutainer #11 Royal Blue Vacutainer Laboratory: Toxicology Additive: 1. None - red stripe 2. EDTA - blue/lavender stripe Inversion Amount: 8x Yields: 1. Red stripe - serum 2. Blue stripe - plasma Common Tests: PB poisoning, heavy metal poisoning Additi
Created by: user-1767416
 

 



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