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Lab Questions
| Question | Answer |
|---|---|
| What is the total magnification of your microscope when you are observing stained bacteria with the oil immersion lens? | 1,000x |
| How can the refraction of light be reduced as it goes through the glass slide and into the lens? | Putting the immersion oil on the stained glass or wet mount and lowering the lens into it. |
| When viewing stained slides, which lens do you use first to focus the microscope? | The low power 10x lens |
| What must you do before storing your microscope after using the oil immersion lens? | Remove the oil from the lens by carefully wiping the lens with several flat pieces of lens paper |
| How can you distinguish true motility from other movement? | Cells are swimming in random directions, once evaporation at the edge of the coverslip causes convection currents to form. |
| How do cells appear when the Brownian movement is causing their motion? | Jiggles in liquid, but if it is motile, then it moves from one point to another. |
| How do cells appear when convection currents are causing their motion? | Moving along in a stream flowing to the edge of the coverslip |
| What is the definition of pure culture? | A population of cells descending from the growth of a single cell and free from contamination. |
| Why is sterile technique important? Give two reasons. | Prevents contamination of our culture with organisms from the environment, and to prevent the culture from contaminating us or others. |
| What is the purpose of a streak plate? | Isolate a colony formed by a single cell from a mixture containing millions of cells. |
| Why is it important to avoid digging into the agar with the loop? | Colonies may come into contact with each other |
| What is the purpose of transferring sterile broth from one tube to another tube of sterile broth? | To prevent the entry of microbes from the environment |
| How did you make sure there are no organisms on your wire loop? | Incinerate the wire loop for 10 secs before and after use. |
| When you increase the magnification, is it necessary to increase or decrease the amount of light? | It is necessary to increase and decrease the amount of light until the object is in focus |
| When looking at unstained material, do you need more or less light than what it is needed to view a stained preparation? | Less light is needed to increase the contrast between cells and liquid. |
| Why is it convenient to have a parfocal microscope? | Allows a slide to be in focus when rotating objective lenses. |
| Why couldn't you see a virus with your microscope even if you increased the eyepiece lens magnification to 100x? | A virus is too small to be seen in a light microscope. |
| When observing a specimen through the microscope, how do you calculate the total magnification? | Objective lens x eyepiece lens |
| Why should you carry the microscope in the upright position? | To prevent the eyepieces from falling out |
| What is the only material that can be used to wipe a lense? | Lense paper |
| Why must you wipe off the eyepiece lens before storing the microscope? | So we don't etch the glass. |
| High dry lens? | 40x |
| Low power lens? | 10x |
Created by:
Viridescent