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Blood Bank

Enhancement Media / Potentiators

QuestionAnswer
increases the dielectric constant, which REDUCED THE ZETA POTENTIAL Protein media
causes agglutination by adjusting ZETA POTENTIAL between red cells 22% Albumin
incubation for 22% albumin 37C for 15-30 mins
reduces FALSE-POSITIVE reactions, more effective than albumin polyethylene glycol (PEG)
incubation for PEG 37C for 10-30mins
decreases the IONIC STRENGTH of a reaction medium and so reduce the zeta potential; increase rate of antibody uptake during sensitization Low Ionic Strength Solution Media (LISS)
incubation for LISS 37C for 5-15mins
enzymes that ENHANCES antibody reactivity to antigens Rh, Kidd, P1, Lewis and I antigens
enzymes that DESTROYS reactivity to red cell antigens Fya, Fyb, M, N, S antigents
enzyme from fig plant Ficin
enzyme from pig stomach trypsin
enzyme from pineapple Bromelin
enzyme from papaya papain
designed to determine of RBCs are COATED WITH AB OR COMPLEMENT OR BOTH Anti-Human Globulin (AHG)
can determine if RBCs have been sensitized with IgG antibody or complement (C3b or C3d) or both Polyspecific AHG
react ONLY with RBCs sensitized with IgG or complement Monospecific AHG
pool of heterogenous anti-IgG from many RABBITS Polyclonal
pool of anti-IgG from a single clone of plasma cells; prepared by HYBRIDOMA TECHNOLOGY; uses MICE Monoclonal
Created by: CohenJanie
 

 



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