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Ch 9 Studyguide
Chapter 9 Studyguide self-tests
| Question | Answer |
|---|---|
| DNA cutting enzymes that often form sticky ends | Restriction enzymes |
| A self-replicating DNA molecule used as a carrier to transmit a gene from one organism to another | Vector |
| An enzyme that links short pieces of DNA into longer pieces | DNA ligase |
| An enzyme that links nucleotides to form DNA | DNA polymerase |
| Probably the most common cloning vector used in genetic engineering | Plasmid |
| The reshuffling of genes between two DNA molecules forms DNA of this type | Recombinant |
| The kind of DNA synthesized by using mRNA as a template | Complimenatary |
| DNA exchange between cells in this process uses polyethylene glycol to improve efficiency | Protoplast fusion |
| Short segments of single-stranded DNA used to recognize a DNA sequence in a gene | Probe |
| In the blue/white screening procedure, the foreign DNA is in these colonies | White |
| These stretches of DNA of eukaryotes do not code for proteins | Intron |
| Sticky ends stick to each other by complementary stretches of single-stranded DNA by ______ pairing. | Base |
| A collectionof bacterial clones each containing a different DNA fragment is called a ________________. | Gene Library |
| To isolate a fragment of DNA containing a gene, DNA fragments of clones are separated by gel electrophoresis. This is an early step in the ____________ technique for DNA analysis | Southern Blot |
| The procedure by which billions of copies of a sequence of DNA can be made in a few hours is called the _________________ reaction | polymerase chain |
| The most elegant way of introducing recombinant DNA into a plant cell is by means of the ____ plasmid, carried naturally by Agrobacterium tumefaciens. | Ti |
| The Human Proteome project has the goal of identifying all the _______ produced by human cells. | proteins |
| The full term is restriction fragment length _____________. | Polymorphisms |