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Non-Blood Form3
| Question | Answer |
|---|---|
| Why is it necessary to make sure that specimens collected for microbiology are not contaminated? | • Poorly collected, contaminated samples may result in the failure to isolate the causative organism • It could lead to inappropriate treatment of the patient. It could be of no clinical benefit to the patient/the patient would not get well. |
| Explain the reason for the abstinence period before collection of semen | • The period of abstinence has an effect on semen parameters, ( • Especially the volume • And the sperm concentration • The fixed period of 2-7 days is primarily to standardize the conditions for evaluation • Reduces inter-sample variations |
| If a semen specimen is produced away from the lab, what are the specimen transportation requirements? | • the specimen must be delivered to the laboratory within 30 minutes of collection • at body temperature during this period |
| Define the term sputum | Mucous or phlegm that is ejected from the lungs, bronchi and trachea through deep coughing |
| When obtaining a rectal swab, the swab should be inserted: | 1-3 cm past the anal sphincter (in the rectum) |
| Give two main reasons for semen analysis. | • To assess fertility • Check the effectiveness of sterilisation Post vasectomy • Forensic reasons |
| Give 3 indications why a sputum specimen may be requested | • Bacterial or viral infection • To assist in the diagnosis lower respiratory tract infections like Tuberculosis • For cytology |
| What is the most important info to write down when labelling the specimen for Ear swabs | Specific site on label-e.g. Right or Left ear |
| What additional instructions would you give the patient that need to submit sputum for TB? | • An early morning specimen on 3 consecutive days is needed. • Label the specimen with the date and day of collection, and return each specimen to the lab preferably on the day it was collected |
| Describe the container used for semen collection | The sample container must be a clean, sterile, wide-mouthed, plastic container with a screw top. The container should be non-toxic to spermatozoa |
| Why can't Sputum for cytology be preserved in 50% alcohol | The alcohol causes mucolytic changes |
| Explain what needs to be checked when receiving a sputum sample | •ensure it is sputum + not saliva •Check label completed •Check if sterile container •Sufficient sample for test •Check if toothpaste/ mouthwash used before collection •Date + time of collection •antibiotic s •Check food particle contamination |
| Describe the method of semen collection requirement as stipulated in the SOP | Collect entire sample by masturbation only |
| What needs to be checked when receiving a rectal swab | That there is stool/feces on the swab |
| Provide the rationale for the following instructions for the collection of a sputum specimen: First morning specimens are preferred | Secretions collect in larger volumes in lungs overnight |
| Which test requires a refrigerated stool specimen? | Faecal fat |
| Which test requires a stool specimen on ice? | Clostridium difficle |
| Which test requires a stool specimen covered in foil? | Stool for Porphyria |
| Answer True or False to the following : Throat swab specimens are most commonly used to aid the diagnosis of streptococcal “strep” infections | True |
| What specimen relevant information must be noted on the requisition form before sending the semen specimen to the laboratory | • Period of abstinence • How the specimen was produced (masturbation) • Date and time of collection • Any difficulties or incomplete sample. • Any recent medication and illnesses during the last 3 months |
| Answer True or False to the following : Approximately 3-5ml of sputum is required for testing | True |
| Name the bacterial throat infection that can result in rheumatic heart disease | Streptococcal |
| What mechanical effect does “saying Ahh” have on the uvula | Raises the uvula |
| Answer True or False to the following : Having the patient say “Ahh” during a throat swab collection drops the uvula | False |
| Describe the instructions for semen collection | •Abstain sexual activity for2-7days before •Wash hands+pass urine •Collect entire sample by masturbation in sterile container •Dont use condom/Coitus interruptus •Dont use other container •Deliver to lab in 30min at body temp •Do questionnaire |
| Answer True or False to the following : Transporting swabs to the lab as soon as possible for testing prevents overgrowth of normal flora | True |
Created by:
cisca