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Molecular Bio Final

QuestionAnswer
What is a natural substrate used for DNA replication using DNA Pol III? dNTP
What is not in the replication fork? Protease
The majority of new nucleotides are added by____? DNA Pol III
Which of the following ions can be found in active site of DNA Polymerase III enzyme? Mg and Zinc
Which of the following is false? The function of the metal ions in DNA polymerase catalytic pocket is? Stabilize the template DNA
Which of the following amino acid residue of DNA polymerase play a critical role in the catalytic site of the DNA Pol during DNA replication by stacking with base of incoming nucleotide? Tyrosine
DNA Polymerase enzyme cannot catalyse DNA synthesis: -in the absence of 3'OH, in the absence of a primer, in 3' to 5' direction All of the above
For DNA replication in eukaryotic cells, which of the following enzyme is not involved? DNA polymerase
DNA replication initiator interact with which of the following sites? Binding site of a replicator
Which of the following is not a component of a replication fork? Reverse transcriptase
Which of the following is unrelated to telomere synthesis or stability? primer synthesis
Which of the following is not a component of a replicator? Polymerase III recruiting site
Which of the following is not true? Seq A is an important regulator in DNA replication fidelity control
To initiate DNA replication in E. coli, which of the following characteristics of protein DNA-A is not true? Binding with Okazaki fragments
Which of the following is true relating to primer proteins? "priming proteins" acts as a primer for DNA synthesis by providing a OH group
McM2-7 is a major player during DNA replication initiation, which of the following is true? Its is a helices
Which of the following is false about Cdk, which is involved in the regulation of replication initiation? Cdk level is high in G1 phase
Which of the following statements about telomerase is false? Its RNA contains 10 times of the repeats of the telomere sequence
Which of the following DNA polymerase enzymes is not involved in eukaryotic cell DNA replication? Pol Beta (B)
Which of the following is used for site specific genome editing before the discovery of CRISPR/Cas9 system? TALEN (Transcription activator-like effector nuclease)
What of the following is true relating to Folk nuclease? Folk nuclease is important for the TALEN system in genome editing
Which of the following is false relating to CRISPR? When CRIPSR system provides acquired resistance against bacterial phage, Cas9 is the only nuclease used for defence
Which if the following is true relating to CRISPR? CRISPR/Cas9 system can be used to target multiple sties of genome for mutations because we can provide multiple guide RNAs
In the CRISPR/Cas9 base editing system, DNA nick activity of the enzyme is important because ____? It guides DNA repair on which of the two DNA strands
To create the first generation of base editor (BE1), a ___ is fused with a mutated Cas9 called dCas9 Cytidine deaminase
Which of the following is not used in genome editing? Regular DNA restriction enzymes
Which two small RNA molecules are critical to the CRISPR/Cas9 system to work in bacteria? crRNA and tracrRNA
Cas9 is a ___? RNA dependent DNA nuclease
If the cells failed to repair the errors introduced by the DNA replication, which of the following DNA repair systems had problems? Mismatch repair
If xeroderma pigments symptoms are observed in a person, which of the following DNA repair systems may have a problem? Nucleotide excision repair
Put the following steps in the correct order 3,1,4,2,5
Which process requires a cell to distinguish between a parental strand and a daughter strand of DNA? Mismatch repair
Which of the following is false relating to DNA photolyase? DNA photolyase is the primary mechanism used for DNA repair
What is the repair template during base excision repair? The strand complementary to the damaged DNA
If damage occurs to the actively transcribed actin gene in a muscle cell____ It would be repaired quickly because of the transcription coupled repair system
Which of the following is false transverion mutation? C to T
Which of the following is a false translation mutation? A to C
For mismatch repair, which of the following protein recognise the mismatch repair? Mut S
Which of the following is unrelated to direct reversal repair? Ligase
Which of the following is a true statement about the translation synthesis repairing? It requires polymerase switching
Which of the following eukaryotic protein has a similar function to E. coli RecA? Rad 51
Which of the following is is true relating to RecA? RecA forms filament and is essential for strand invasion
Which of the following is true relating to demethylase medicated direct reversal? Demythlase mediated direct reversal DNA repair is rarely used for DNA repair
Which of the following is true? SPO11 is a DNA polymerase
One mechanism of gene conversion is Synthesis-dependent strand annealing (SDSA), which of the following statements is false about SDSA? Replication fork is not related to SDSA
Which of the following is the first step of homologous recombination? Alignment of two identical or nearly identical DNA molecules
In the RecBCD pathway of homologous recombination, which of the following statements is false? Chi site is rare in the genome
Which of the following proteins is not present in the Pre-RC in eukaryotic genome replication? DNA polymerase
The function of topoisomerase in genome replication is ? Both A and B: to release tension of supercoil and to disentangle circular genome
In a human chromosome, the telomere sequence is repeated.... hundreds to thousands of times
If you detected A and T only in a short piece of nucleic acid molecule, the molecule ____ can be DNA
If a DNA fragment contains 105 bps, which of the following is true? The diameter of the DNA helix is about 20 Angstrom
Which of the following statements is false relating to DNA? The two strands of a DNA helix are identical in nucleotide sequence
Which one of the following proteins is correct? Major groove present the information of the DNA sequence to interacting partners
Which of the following is important for providing the DNA sequence information to other cellular partners? All the three groups in other answers are important: proton receptors, proton donors, and methyl group
Which of the following is correct relating to minor grooves? Minor groove doesn't provide enough information to reveal the DNA sequence
In a nucleotide such as dATP, the base is attached to the ____ carbon of deoxyribose 1'
Which of the following is false relating to U pairing? U can't pair with G in RNA
The histone H2A and H2B form dimers in the cell
The DNA fragment wrapping around the histone core of a nucleosome is about 147 bp
Centromere specific histone protein CENP-A is H3 Variant
The bromodomain protein usually binds with ______ acetylated histones
Which of the following statement about nucleosome remodelling complex is false? Nucleosome remodelling complex is usually a single large protein
Which of the following is true about DNA methylation in insects? In insects, DNA methylation occurs frequently in the coding region of a gene
Royal jelly is likely to _______ reduce DNA methylation in selected genes
Which of the following process is not involved in X chromosome inactivation in mammals? Xist RNA translation
The X chromosome inactivation (on the silenced chromosome) in mammals involved following steps in order: Xist RNA expression, Xist RNA coding of the selected X chromosome, H3 lysine 27 methylation, and DNA methylation
What is the central dogma of molecular biology? DNA Replication, transcription, and translation
What four bases pair with RNA? A,G,C,U
What four bases pair with DNA? A,G,C,T
How is RNA different from DNA? Is a ribose sugar, has no one-to-one interactions, a complex tertiary structure, is more flexible than DNA)
What is an euchromatin? Dark staining, densely packed DNA
What is a heterochromatin? Light staining, loosely packed DNA
What is X chromosome inactivation? When one pair of the X chromosomes on each cell is inactivated: dependent on Xist ncRNA and involved DNA methylation and histone modification
What DNA polymerases are involved with prokaryotes? DNA Pol I and DNA Pol III
What DNA polymerises are involved with eukaryotes? Pol a, Pol δ, and Pol ϵ
What is the function of DNA Pol I? degrades primer, fills in gaps left by RNA primer removal
What is the function of DNA Pol III? Chromsome replication
What is the function of DNA Pol a? Primer synthesis, nucleotide and base excision repair
What is the function of DNA Pol δ? Lagging-strand DNA synthesis, nucleotide and base excision repair
What is the function of DNA Pol ϵ ? Leading-strand DNA synthesis, nucleotide and base excision repair
What metal ions are involved with DNA Pol? Mg and Zinc
What amino acids are involved with DNA Pol? Tyrosine, arginine and lysine
What is the role of tyrosine in relation to DNA polymerase? Encourages "base-stacking"
What is the roles of argine and lysine in relation to DNA polymerase? Stabilises pyrophosphate
What are cyclins and what are their roles? Proteins that control cell progression through cell cycle; includes Cdk
Eukaryotic mRNAs are synthesised by? RNA Polymerase II
Which of the following is more likely to be the -10 region sequence of the σ70 promoters? TATAAT
Which of the following is usually far away from the -10 consensus sequence of a promoter in position? UP element
There are five channels in the prokaryotic RNA polymerase holoenzyme. During isomerization, the protein σ1.1 fragment moves out of the ______ channel. Downstream DNA entrance
The important phosphorylation sites of the CTD of RNA Pol II are? Ser 2 and Ser 5
Created by: 3325496134387138
 

 



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