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bio exam review pt2
| Question | Answer |
|---|---|
| How does bacteria grow by binary fission? | single DNA molecule replicates and both copies attach, at different points, to the cell membrane. |
| The relationship between growth rate and doubling/generating time | the rule of 70 |
| How many cells will be found in a culture growing exponentially, | 2 cells, then 4 cells, then 8 cells, then 16, then 32 |
| The different direct and indirect approaches can be used to measure/quantify bacterial growth | Direct - measurement of the growth of microbial populations through measuring cell numbers, measuring the populations total mass. Indirect- not always necessary to count microbial cells to estimate their numbers |
| the name we give a microbe to describe its temperature range and optimum | Mesophilic |
| the name we give a microbe if given its pH and optimum | neutrophiles, acidophile, and alkaliphile |
| the name we give a microbe if given its %NaCl range and optimum | nonhalophiles, nonhalotolerant, halotolerant |
| the macromolecules that the major six elements (C,H,O,N,P,S) are found in | carbohydrates, lipids, necleic acids, and proteins |
| which elements are considered essential | carbon, hydrogen, oxygen, nitrogen, phosphorus, sulfer |
| 3 methods by which bacteria can take up foreign DNA | transformation, transduction, conjugation |
| what happens during transcription What is the major enzyme involved | he DNA sequence of a gene is copied into RNA RNA polymerase is involved |
| what happens during translation? | mRNA sequence is read using the genetic code. |
| during translation, what cell structure carries out this process | ribosomes |
| ribosomes in prokaryotes vs eukaryotes | Prokaryotic ribosomes are composed of a small RNA molecule, called a messenger RNA, and a protein called a ribosomal protein. Eukaryotic ribosomes, are composed of a large RNA molecule, called an mRNA, and a number of different proteins. |
| 3 types of RNA an how they are involved in translation | mRNA- messenger rRNA- ribosomal tRNA- transfer |
| what happens during DNA replication | is the process by which the chromosome copies itself |
| what major enzymes are involved in DNA replication | amylase, lipase, DNase, gelatinase |
| are plasmids found mostly in prokaryotes or eukaryotes | eukaryotes |
| what types of genes are found on plasmids | TRA gene |
| how are chromosomes and plasmids different | plasmid is a circular double-stranded extra-chromosomal DNA structure of bacteria while the chromosome is a well-organized thread-like structure that contains genomic DNA tightly coiled with proteins. |
| differnece between prokaryotic and eukaryotic genomes | prokaryotic genome is present in the cytoplasm while eukaryotic genome confines within the nucleus |
| anaerobic electron acceptor | anaerobic respiration uses an alternative terminal electron acceptor |
| difference between exoenzymes and endoenzymes | exoenzymes- catalyze reactions outside of the cell endoenzymes-function within the cells |