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Dehydration

ICLS

QuestionAnswer
the process of removing intercellular and extracellular water from the tissue following fixation and prior to wax impregnation Dehydration
Most common dehydrating agent Alcohol
Enumerate the dehydrating agents 1. Alcohol (most common) 2. Acetone 3. Dioxane 4. Cellosolve 5. Triethyl Phosphate 6. Tetrahydrofuran
T or F Under no circumstances a formalin-fixed tissue should be transferred directly to higher grades of Alcohol T
T or F Concentrated Alcohols tend to harden only the surface of the tissue while the deeper parts are not completely penetrated. T
A temperature of __________ will hasten dehydration time and is especially used for tissue sections that require urgent examinations such as fragmentary biopsies. 37 degrees Celsius
the alcohol recommended for routine dehydration of tissues. It is considered as the best dehydrating agent because it is fast-acting, it mixes with water and penetrates tissues easily. Ethyl Alcohol
is a toxic dehydrating agent, primarily employed for blood and tissue films and for smear preparations. Methyl Alcohol
is utilized in Plant and Animal Micro-techniques, and a slow dehydrating agent. Butyl Alcohol
to ensure complete dehydration, a layer of _____, about __-inch-deep is placed in the bottom of the container and covered with filter paper. A blue discoloration of Copper Sulfate crystals will indicate full saturation of dehydrating fluids with water. 1. Anhydrous Copper Sulfate 2. 1/4 inches
CHARACTERISTICS OF AN IDEAL DEHYDRATING AGENT 1. Dehydrate rapidly CHARACTERISTICS OF AN IDEAL DEHYDRATING AGENT 2. Should not evaporate fast 3. Should dehydrate fatty tissues 4. Should not harden tissues excessively 5. Should not remove stains 6. Not toxic
 

 



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