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BACTERIOLOGY
| Question | Answer |
|---|---|
| Decolorizer of Auramine Rhodamine | 0.5% acid alcohol |
| Color or acid fast in auramine rhodamine | Yellow fluorescence |
| Auramine rhodamine quenching agent | 0.5% potassium permanganate |
| Iodine + QUATS (detergent) | Iodophor |
| Iodine + 75% ethanol | Tincture of iodine |
| MacFarland standard | 99.5 mL: 1% H2SO4 0.5 mL: 1.175% BaCl2 |
| Yellow colonies in TCBS | V. cholerae, V. alginolyticus (Sucrose fermenters) |
| Green colonies in TCBS | V. parahemolyticus, V. vulnificus (Non sucrose fermenter) |
| Culture media for Brucella | Castañeda broth, W medium |
| DOES NOT GROW ON MAC | HACEK, C. meningospeticum, Francisella, Pasteurella |
| Hockey puck, wagon wheel | Moraxella catarrhalis |
| Fisheye on EMB | Enterobacter |
| Swarming | Proteus mirabilis, Clostridium tetani, Clostridium septicum |
| Wrinkled, dry | Nocardia, Neisseria sicca |
| Star shaped | Aggregatibacter actinomycetemcomitans |
| Rosette | Cardiobacterium hominis |
| Mercury droplets / shiny half pearl | Bordetella pertussis |
| Fluorescence red | Porphyromonas, Veilonella, Prevotella |
| Nutritionally variant streptococci | Abotrophia, Granulicatella (Pyridoxal (Vit B6), Thiol) |
| Friedlander’s bacillus | Klebsiella pneumoniae |
| Triple negative LOA (- - -) | Pantoea agglomerans |
| Osteomyelitis in motorcycle accidents | Serratia plymuthica |
| Parathyphoid fever | Salmonella paratyphi A and B |
| Triple positive LOA (+ + +) | Plesiomonas shigelloides |
| Guillain Barre syndrome | Campylobacter jejuni, Chlamydia pneumoniae |
| Y. enterocolitica is motile at | 22 C |
| Y. pseudotuberculosis is motile at | 18-25 C |
| “Pseudoinfections” | Stenotrophomonas maltophilia |
| Pffeifer’s bacillus | Haemophilus influenzae |
| For fastidious organisms | Enriched media: BAP, CAP |
| Visualizes metabolic differences of bacteria | Differential media: MAC, BAP, EMB, HEA |
| Brucella abortus growth requirement | Erythritol, Increase humidity |
| Eaton agent | Mycoplasma pneumoniae |
| Lumpy jaw, Sulfur granules | Actinomyces israelli |
| Blue milk | Pseudomonas syncyanea |
| Yellow milk | Flavobacterium synxanthum |
| Blue green milk | P. aeruginosa |
| Red milk | S. marcescens |
| Souring of milk | Streptococcus lactis |
| Proteolytic action on coagulated milk | B. subtilis |
| Slimy or ropy milk | Alcaligenes viscosus |
| 0.1 fuchsin replace ___ for GS of Legionella | Safranin |
| Hippurate hydrolysis (+) | S. agalactiae, L. monocytogenes, C. jejuni, G. vaginalis |
| Double zone of hemolysis | C. perfringens |
| Extensive branching/aerial hyphae, LYSOZYME RESISTANT | Nocardia |
| Tap water agar (+), Filamentous bacteria | Streptomyces |
| Poly-D-glutamic acid | B. anthracis |
| Gold standard for rabies | Direct fluorescent antigen |
| Test for resistance to penicillin | Beta lactamase test |
| Organ for locomotion of spirochetes, flagella-like | Axial filaments / Axial fibrils |
| Non treponemal test | RPR, VDRL, TRUST |
| Infectious jaundice | Leptospirosis |
| Hallmark of anicteric phase of Leptospira | Aseptic meningitis |
| Weil’s disease | Icteric phase |
| Halberstadter-prowazeik (glycogen inclusion bodies) | Chlamydia trachomatis |
| Levinthal-Cole-Lillie (non glycogen inclusion bodies) | Chlamydia psittaci |
| Causative agent of TWAR | Chlamydia pneumoniae |
| Mycoplasma requirement for growth | Sterol |
| Large fried egg colonies | Mycoplasma hominis |
| Tiny fried egg colonies | Ureaplasma urealyticum |
| Subtype of C. trachomatis that causes uti, cervitis, urethritis, epididymitis | D to K |
| Subtype of C. trachomatis that causes lymphogranuloma venereum or buboes | L1,L2,L3 |
| Rickettsia cross react with | Proteus |
| Gold standard for RABIES | Direct Fluorescent Antigen |
| Epidemic typhus, Sporadic thypus | R. prowazekii |
| Endemic typhus, Murine typhus | R. typhi |
| Scrub thypus | R. tsutsugamushi |
| Trench fever | Bartonella quintana |
| Culture media of C. diptheriae that inhibits normal flora | Potassium tellurite |
Created by:
alyanaRMT