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BIO 181 Exam Prep
| Question | Answer |
|---|---|
| 1. Which enzyme unwraps the double helix? | Helicase |
| 2. In the ribosome, what pairs with the codon? | Anticodon |
| 3. List the two basic categories of nitrogenous bases in nucleic acids: | Purines and Pyrimidines |
| 4. During mRNA maturation, what is the name of the part of the mRNA that is removed? | Intron |
| 5. During DNA replication in the lagging strand there is production of this component? | Okazaki fragment |
| 6. There are two types of protein synthesis in eukaryotic cells. These two types can be distinguished because the polyribosome is either | free or membrane-bound |
| 7. Assume that you have a eukaryotic cell with three chromosomes with it. How many 5' ends would there be? | Gap1: 6, Gap2: 12 |
| 8. List one type of post-translational modification. | Proteolysis |
| 9. RNA synthesis, also called RNA transcription occurs in three phases. List them from beginning to end in order. | Initiation, elongation, termination |
| 10. Uridine is a nucleotide in DNA. True or False? | False |
| 11. Which sugar is present in RNA? | Ribose |
| 12. The double helix of DNA is wrapped about 1¾ times around which of these components? | Nucleosome |
| 13. How many hydrogen bonds are there between an A and its paired nucleotide? | 2 |
| 14. The DNA polymerase that replicates the parental strand of DNA reads the parental DNA from 5’ to 3” and makes a new DNA strand from 3’ to 5’. True or false? | False |
| 15. Replication of DNA is semi-conservative. True or False? | True |
| 16. Draw the sugar in DNA and label the carbons as discussed in the lecture. Then circle the carbon that is different in RNA. | See image |
| 17. Which strand is produced more rapidly? | Leading Strand |
| 18. Which enzyme is used first to permit DNA polymerase III to begin replicating the 3’ strand? | Primase |
| 19. RNA is double stranded. True or False? | False |
| 20. In DNA, what does the nucleotide labeled as A pair with? | Thymine(T) |
| 21. In the lagging stand, the DNA is read from the parental DNA’s 3’ to 5’ end. True or False? | True |
| 22. In eukaryotic cells the start codon for protein synthesis is | AUG, code for methionine |
| 23. How many "stop" codons exist? | 3 |
| 24. Protein synthesis changes from the language of _ to the language of _ | nucleotides; amino acids |
| 25. In the ribosome, what is the name of the bond that links one amino acid to the next? | Peptide Bond |
| 26. For DNA Polymerase III to work which of these components has to act first? | Primase |
| 27. Which nucleotide is different in RNA compared to DNA? | Thymadine |
| 28. DNA replication in eukaryotes is sped up by multiple origins of replication. True or False? | True |
| 29. Strands of the backbone of DNA are parallel. True or Fasle? | False |
| 30. The genetic code for the conversion of mRNA codons to proteins has redundancy. True or False? | True |
| 31. During DNA replication is greatly reduced by enzymes that proof read the newly replicated DNA. True or False? | True |
| 32. List three items required for photosynthesis to occur | Water, Sunlight, CO2 |
| 33. The antenna system is in the _ membranes | Thylakoid |
| 34. The most abundant protein in the world is called | Rubisco |
| 35. The light reactions produce _ and_ and this process also results in the release of _ | ATP and NADPH + H+; Oxygen |
| 36. The Calvin-Benson cycle takes in _ and _ from the light reaction and also __ __ to make carbohydrates | ATP and NADPH + H+; carbon dioxide |
| 37. Specifically in a cell where does Glycolysis occur? | Cytoplasm |
| 38. FADH2 results as one of the products of the citric acid cycle. True or False? | True |
| 39. Glycolysis requires the investment of two _ in order to obtain a gross output of four _ | ATPs, ATPs |
| 40. The cell does not need ATP to survive. True or False? | False |
| 41. Specifically where in a cell does the citric acid/Kreb's cycle occur? | Mitochondrial Matrix |
| 42. The citric acid/Kreb's cycle breaks down carbohydrates into CO2. True or false? | True |
| 43. Where is carbon dioxide produced? | Mitochondrial Matrix |
| 44. Mast cells release histamine. True or False? | True |
| 45. Does gravity have a role in some part of the lymphatic fluid flow back to the circulatory system? | Yes |
| 46. Inflammation is caused by an increased blood supply due to __ released by some cells of the immune system. | Histamine |
| 47. Having a fever of 100F can help you fight off infections. True or False? | True |
| 48. The humoral immune system involves cells in the immune system. True or False? | True |
| 49. How does the lymph fluid return from the legs to the circulatory system? | By muscles that contract when moving |
| 50. In 1 mL of blood, there are 1.5 million white blood cells. True or False? | False |
| 51. Red blood cells originate from the bone marrow. True or False? | True |
| 52. A bacterium coated by antibodies is a signal that a macrophage should endocytose (eat) it. True or False? | True |
| 53. When a B cell is shown an antigen on a class II major histocompatibility complex (MHC) protein it will go through ______________ ______________ and B cells subsequently can become ______________ and/or ______________ ______________. | Blast transformation; Plasma cells; memory cells |
| 54. Lymph nodes are a place where cells of the immune system accumulate and share information. True or False? | True |
| 55. You should have your appendix removed whenever possible. True or False? | False |
| 56. Bone marrow is a site where mature immune cells are found. True or False? | False |
| 57. If a macrophage endocytoses (i.e., eats) an antigen it can digest it and present it on a ______________ Major histocompatibility complex protein to a ______________. | Class II; T-Cell |
| 58. When immune cells mature and can recognize trans-membrane proteins indicating “self”, this is called ______________. | Tolerance |
| 59. Memory cells “remember” the antigen and make a faster and greater response the next time the antigen is encountered. True or False? | True |
| 60. Any cell can present an antigen on a class I major histocompatibility complex (MHC) protein. True or False? | True |
| 61. Any cell can present an antigen on a class II major histocompatibility complex (MHC) protein. True or False? | False |
| 62. Red blood cells are a part of the immune system. True or False? | False |
| 63. B and T cells are called lymphocytes. True or False? | True |
| 64. In the lecture we discussed normal flora, this refers to: | Bacteria and fungi on the surface of the body that compete against pathogens and can produce substances toxic to pathogens |
| 65. Lymphatic tissue is composed of the following five components. Please list them. | Bone Marrow, Thymus, Spleen, Lymph Nodes, Lymphatic Ducts |
| 66. There are times when the spleen can be considered to be the primary lymph node. True or False? | True |
| 67. Natural killer cells are part of the specific immune system. True or False? | False |
| 68. Epithelial cells serve as a barrier to prevent non-specific movement of chemicals from one side of the epithelium to the other side. True or False? | True |
| 69. Adhering junctions are based on two different types of cytoskeleton filament systems. Name those systems. | Actin filament, intermediate filament |
| 70. The molecular seal that serves as the barrier is called a _____________ _____________. While this is a good barrier, such barriers are not very strong and must be underlined by _____________ _____________. | tight junction; adhering junction |
| 71. What is bile made from? And how does oatmeal reduce cholesterol levels? | Bile is synthesized from cholesterol. Oatmeal is a high fiber source that binds with bile and decreases it absorption by the ileum |
| 72. Name the main enzyme in the stomach. | Pepsin |
| 73. How does the pancreas neutralize the pH of the chyme that comes from the stomach? | The pancreas produces a secretion rich in bicarbonate ions which neutralizes the pH of the chyme |
| 74. When glucose is taken in by the digestive track and enters your blood, cells are triggered to take in the glucose by what mechanism? | Insulin |
| 75. When amino acids are acquired by food because your body can’t make them they are called: | Essential amino acids |
| 76. Vitamin K cannot be made by the cells of your body. Where is it made? | It is made in our gut by bacteria |
| 77. Think about feedback loops. What triggers the gall bladder to secrete bile? | The body detects fat content in ingested food and sends a signal to the gall bladder to constrict and create bile into the duodenum |
| 78. If something is called a vitamin, it cannot be made by the organism and must be taken in as a part of diet. True or False? | True |
| 79. In what way is the making of a primary lysosome similar to the enzymes delivered by the pancreas? | Both release enzymes and break down sugars, fatty acids, nucleic acids, and proteins |
| 80. Name the sphincter that separates the stomach from the small intestine. | Pyloric sphincter |
| 81. Draw concentric circles and label the four layers of the gut. Also label the hole at the inner most circle. List what is in these four layers. | Mucosa, submucosa, muscular, and serosa |
| 82. List the layers of the digestive track covered by a thick layer of mucous. | Mucosa, submucosa |
| 83. 90% of absorption in the intestine occurs where? | The jejunum and the ileum |
| 84. The digestive track begins with the __ and the first enzyme added is __ | Mouth, amylase |
| 85. The esophagus has two distinct muscles that close off the tube. What are these called? | Circular and longitudinal muscles |
| 86. Write a few sentences to distinguish between digestion and absorption. | Digestion is the breaking down of foods into smaller chunks. Absorption is taking in nutrients into the body |
| 87. List the order in which storage compounds are utilized if there is no intake of food. | Glycogen, fat, protein |