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NHA order of draw
| Question | Answer |
|---|---|
| What are standard phlebotomy supplies | Gloves Tourniquet Isopropyl alcohol wipes Non-alcohol prep kits or swabs Non-sterile gauze Typically 2 x 2 sizes avoid cotton balls Conhesive wrap or paper tape Double pointed needles Butterfly needle Blood collection tube Plastic or glass capillary tub |
| Why is the order of draw important | The tubes must be drawn in proper order to avoid cross-contamination of the additives if the tubes are not drawn in the correct order the additives “inadvertently affect the test |
| What is order of draw | Yellow (blood cultures) Light blue Red Gold or the tiger top Green Lavender Gray |
| Additive for gray And used for what test | Potassium oxitate and sodium fluoride Glucose testing (GTT) |
| What is the additive for lavender what test is lavender used for | EDTA (anticoagulant) Whole blood hematology determinations, routine immunohematology testing And Blood donor screening |
| What additive and test is GREEN top | Sodium heparin Lithium Heparin For plasma determinations in chemistry |
| Gold or tiger top (red grey) | Serum separator tube (SST) Clot activator Thixotropic gel (creates a barrier) For serum determinations in chemistry May be used for a routine blood donor screening and diagnostic testing of serum for infectious disease |
| RED Top Additive Procedures or lab use | Plastic-clot activator Glass- no additive (plain) For serum determinations in chemistry Maybe use for routine blood donor screening and diagnostic testing of serum for infectious disease |
| Light blue Additive and lab uses | Sodium citrate Anticoagulant Coagulation studies |
| Cultures or culture collections color may vary Yellow | Sodium polyanethol sulfonate (SPS) SPS blood culture specimen collections in microbiology |
| Additional tubes | Micro collection tubes for capillary blood requiring a chemical out of capillary tubes yellow top tubes and vacuum culture vials for a blood cultures |