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ASCP Blood products
| Question | Answer |
|---|---|
| The minimum hemoglobin concentration in a fingerstick from a male donor is | The minimum requirement regardless of sex is 12.5 g/dL (125 g/L) |
| A cause for permanent deferral of blood donation is | History of jaundice of uncertain cause. Jaundice is a sign of liver impairment which may be due to HBV or HCV. Infection with either one is cause for permanent deferral. |
| Would this patient be accepted for blood donation? 32 year old woman who received a transfusion in a complicated delivery 5 months previously. | No, deferral period for transfusion is 6 months |
| Would this patient be accepted for blood donation? 19 year old sailor who has been stateside for 9 months and stopped taking his anti-malarial medication 9 months previously. | No, deferral for travel to areas endemic for malaria is 12 months regardless of antimalarial prophylaxis. |
| Would this patient be accepted for blood donation? 22 year old college student who has a temperature of 99.2°F (37.3°C) and states that he feels well, but is nervous about donating. | Yes, temperature cannot exceed 99.5°F or 33.5°C for donation. |
| Would this patient be accepted for blood donation? 45 year old woman who has just recovered from a bladder infection and is still taking antibiotics. | No, a person taking antibiotics may have bacteremia. |
| Would a recent close contact with a patient with viral hepatitis be cause for permanent rejection status of a donor? | No |
| Would a confirmed positive test for HBsAg 10 years previously be cause for permanent rejection status of a donor? | Yes, a positive test for HBsAg at any time is a indefinite deferral |
| A woman has a spontaneous abortion at 2 months of pregnancy, 3 months previously. Can she be accepted as a blood donor? | Yes, if she has not been pregnant in the last 6 weeks she is able to donate. |
| History obtained from a prospective female blood donor: Age: 16 Temperature: 99.0°F (37.2°C) Hct: 36% History: tetanus toxoid immunization 1 week previously Which results if any, excludes this donor from giving blood? | Hct has to be > 38%. A donor may be 16 unless state law differs. Toxoids and vaccines from synthetic or killers sources have no deferral. |
| What temperature must the donor not exceed in order to donate? | 99.5°F (37.5°C) |
| What must the blood pressure be in order to donate? | <180 mm Hg systolic and <100 mm Hg diastolic |
| What is the minimum platelet count required for frequent repeat donors? | 150 x 10³ /μL ...platelet count is not required for first time donation or if the interval between donations has been at least 4 weeks. |
| Prior to blood donation, the intended venipuncture site must be cleaned with a scrub solution containing what? | PVP iodine complex |
| What must all donor testing include? Hint: it was the first mandated donor screening test for infectius disease | Serological test for syphilis |
| What temperature should platelets be stored for optimum function? | 20°-24°C |
| What is the most common posttransfusion hepatitis? | Hepatitis B |
| What do you test donors for when wanting to detect the most common cause of posttransfusion hepatitis? | HBsAg |
| The Western blog is a confirmatory test for the presence of what? | Anti- HIV-1 |
| What test uses purified HIV proteins to confirm reactivity in samples whose screening test for anti-HIV is positive? | Western blot |
| What test is used to detect donors who are infected with the AIDS virus? | Anti-HIV 1,2 (human immunodeficiency virus types 1 and 2) |
| What is a commonly used screening method for anti-HIV-1 detection? | Enzyme-labeled immunosorbent assay (ELISA) |
| Rejuvenation of a unit of Red Blood Cells is a method used to what? | Restore 2,3 DPG and ATP to normal levels |
| A unit of Red Blood Cells expiring in 35 days is split into 5 small aliquots using a sterile pediatric quad set and a sterile connecting device. Each aliquot must be labeled as expiring when? | The original date of unsplit unit |
| When platelets are store on a rotator set on an open bench top, the ambient air temperature must be recorded when? | Every 4 hours |
| When temperature should fresh frozen plasma be stored and for how long? | -18°C or below for 12 months |
| What is the optimum storage temperature for frozen Red Blood Cells? | -65°C or lower |
| What is the optimum storage temperature for Red Blood Cells? | 1-6°C |
| If the seal is entered on a unit of Red Blood Cells stored at 1°C to 6°C, what is the maximum allowable storage in hours? | 24 hours |
| What is the optimum storage temperature for cryoprecipitated AHF is what? | -18°C or lower |
| Cryoprecipitated AHF must be transfused within what period of time following thawing and pooling? | Within 4 hours |
| Whole blood derived platelets are stored at what temperature? | 20° to 24°C with continuous gentle agitation |
| Platelets prepared by the PRP method may be stored for up to how many days? | 5 days |
| According to AABB standards, Fresh Frozen Plasma must be infused within what period of time following thawing? | Thawed FFP must be stored at 1-6°C for no more that 24 hours |
| Cryoprecipitated AHF maintained in the frozen state at -18°C or below has a shelf life of how long? | 12 months in the frozen state |
| Once thawed, fresh frozen plasma must be transfused within how many hours? | 24 hours |
| What is an important determinant of platelet viability following storage? | PH of platelets should be kept at 6.2 or above throughout the storage period |
| In the liquid state, plasma should be stored at what temperature? | 1-6°C |
| During storage, the concentration of 2,3 DPG decreases in a unit of what? | Red Blood Cells causing a shift to the left in the oxygen dissociation curve. |
| Cryoprecipitate is used primarily for what? | Fibrinogen replacement |
| In what situation will apheresis platelets be irradiated? | A direct donation from mother to son |
| Irradiation of a unit of Red Blood Cells is done to prevent the replication of what? | Donor T lymphocytes |
| Plastic bag overwraps are recommended when thawing units of FFP in 37°C water baths because they prevent what? | The entry ports being contaminated with water |
| Which of the following blood components must be prepared within 8 hours after phlebotomy? | Fresh Frozen Plasma (FFP) |
| Cryoprecipitated AHF contains how many units of Factor VIII? | 80 units in 10 ML of plasma |
| What is the most effective component to treat a patient with fibrinogen deficiency? | Cryoprecipitated AHF |
| Upon inspection, a unit of platelets is noted to have visible clots, but otherwise appears normal. What should the technologist do? | Quarantine and gram stain for culture because clots in the unit may indicate contamination |
| According to AABB standards, Platelets prepared from Whole Blood shall contain a minimum of how many platelets? | 5.5 x 10¹⁰ per unit in at least 90% of the units tested |
| According to AABB standards, at least 90% of all Apheresis Platelets units tested shall contain a minimum of how many platelets? | 3.0 x 10¹¹ |
| What is proper procedure for preparation of Platelets from Whole Blood? | Light spin followed by a hard spin |
| According to AABB standards, what is the minimum pH required for Platelets at the end of the strorage period? | 6.2 |
| Per AABB standards, how should Platelets be stored? | Platelets should be stored at 20°-24°C with continuous agitation and must be separated from whole blood units. |
| A unit of Whole Blood-derived (random donor) Platelets should contain at least how much? | 5.5 x 10¹⁰ platelets |
| Leukocyte-Reduced Red Blood Cells are ordered for a newly diagnosed bone marrow candidate. How should this product be prepared? | Transfuse through a leukocyte-removing filter |
| A father donating platelets to his son is connected through a continuous flow machine that separates Platelets from Whole Blood. As Platelets are harvested, all remaining elements are returned to donor. What is this called? | Apheresis |
| In order to qualify as a donor for autologous transfusion w hemoglobin should be at least what? | 11 g/dL (110 g/L) |
| What are the minimum pretransfusion testing requirements for autologous donations collected and transfused by the same facility? | ABO and Rh only |
| In quality assurance program, cryoprecipitated AHF must contain a minimum of how many international units of Factor VIII? | 80 international units. FDA requires that 4 representatives units be tested for factor VIII levels of 80 IU or higher. |
| An assay of plasma from a bag of Cryoprecipitated AHF yields a concentration of 9 IU of Factor VIII per mL of Cryoprecipitated AHF. If volume is 9 mL, what is Factor VIII content of the bag in IU? | 81 9 IU of Factor VIII is PER mL and volume is 9 mL. 9x9=81 |